Phenotypic and functional characterisation of synovial fluid-derived neutrophils in knee osteoarthritis and knee infection
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61989592%3A15110%2F23%3A73614585" target="_blank" >RIV/61989592:15110/23:73614585 - isvavai.cz</a>
Alternative codes found
RIV/61989100:27240/23:10250541 RIV/00098892:_____/23:10157199
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1063458422008639?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1063458422008639?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.joca.2022.09.011" target="_blank" >10.1016/j.joca.2022.09.011</a>
Alternative languages
Result language
angličtina
Original language name
Phenotypic and functional characterisation of synovial fluid-derived neutrophils in knee osteoarthritis and knee infection
Original language description
Objective: An increase in the number of neutrophils (NEUs) has long been associated with infections inthe knee joints; however, their impact on knee osteoarthritis (KOA) pathophysiology remains largelyunexplored.Design: This study compared the phenotypic and functional characteristics of synovial fluid (SF)-derivedNEUs in KOA and knee infection (INF).Results: KOA NEUs were characterised by a lower expression of CD11b, CD54, and CD64 and higherexpression of CD62L, TLR2, and TLR4 compared with INF NEUs. Except for CCL2, lower levels of inflammatory mediators and proteases were detected in KOA SF than in INF SF. Functionally, KOA NEUsdisplayed increased reactive oxygen species production and phagocytic activity compared with INF NEUs.Moreover, KOA and INF NEUs differed in cell sizes, histological characteristics of the surrounding synovialtissues, and their effects on the endothelial cells assessed by human umbilical vein endothelial cells.When KOA patients were subdivided based on the SF NEU abundance, patients with high NEUs (10%e60%) were characterised by i) elevated SF protein levels of TNF-a, IL-1RA, MMP-9, sTREM-1, VILIP-1 andii) lower CD54, CD64, TLR2 and TLR4 expression compared to patients with low NEUs (<10%). Analysis ofpaired SF samples suggests that low or high NEU percentages, respectively, persist throughout the courseof disease.Conclusions: Our findings suggest that NEU may play a significant role in KOA pathophysiology. Furtherstudies should explore the mechanisms that contribute to the increased number of NEUs in SF and theclinical consequences of neutrophilic phenotype in KOA.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30102 - Immunology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2023
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
OSTEOARTHRITIS AND CARTILAGE
ISSN
1063-4584
e-ISSN
1522-9653
Volume of the periodical
31
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
11
Pages from-to
72-82
UT code for WoS article
000906534300001
EID of the result in the Scopus database
2-s2.0-85144588727