Lif1 SUMOylation and its role in non-homologous end-joining

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F13%3A00060587" target="_blank" >RIV/00159816:_____/13:00060587 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/13:00066405

Result on the web

<a href="http://dx.doi.org/10.1093/nar/gkt236" target="_blank" >http://dx.doi.org/10.1093/nar/gkt236</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gkt236" target="_blank" >10.1093/nar/gkt236</a>

Result language

angličtina

Original language name

Lif1 SUMOylation and its role in non-homologous end-joining

Original language description

Non-homologous end-joining (NHEJ) repairs DNA double-strand breaks by tethering and ligating the two DNA ends. The mechanisms regulating NHEJ efficiency and interplay between its components are not fully understood. Here, we identify and characterize theSUMOylation of budding yeast Lif1 protein, which is required for the ligation step in NHEJ. We show that Lif1 SUMOylation occurs throughout the cell cycle and requires the Siz SUMO ligases. Single-strand DNA, but not double-strand DNA or the Lif1 binding partner Nej1, is inhibitory to Lif1 SUMOylation. We identify lysine 301 as the major conjugation site and demonstrate that its replacement with arginine completely abolishes Lif1 SUMOylation in vivo and in vitro. The lif1-K301R mutant cells exhibit increased levels of NHEJ repair compared with wild-type cells throughout the cell cycle. This is likely due to the inhibitory effect of Lif1 SUMOylation on both its self-association and newly observed single-strand DNA binding activity. Take

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic acids research

ISSN

0305-1048

e-ISSN

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Volume of the periodical

41

Issue of the periodical within the volume

10

Country of publishing house

GB - UNITED KINGDOM

Number of pages

13

Pages from-to

5341-5353

UT code for WoS article

000319806600024

EID of the result in the Scopus database

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