Sumoylation of the Rad1 nuclease promotes DNA repair and regulates its DNA association

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F14%3A00073652" target="_blank" >RIV/00216224:14110/14:00073652 - isvavai.cz</a>

Alternative codes found

RIV/00159816:_____/14:00061058

Result on the web

<a href="http://dx.doi.org/10.1093/nar/gku300" target="_blank" >http://dx.doi.org/10.1093/nar/gku300</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1093/nar/gku300" target="_blank" >10.1093/nar/gku300</a>

Result language

angličtina

Original language name

Sumoylation of the Rad1 nuclease promotes DNA repair and regulates its DNA association

Original language description

The Saccharomyces cerevisiae Rad1-Rad10 complex is a conserved, structure-specific endonuclease important for repairing multiple types of DNA lesions. Upon recruitment to lesion sites, Rad1-Rad10 removes damaged sequences, enabling subsequent gap fillingand ligation. Acting at mid-steps of repair, the association and dissociation of Rad1-Rad10 with DNA can influence repair efficiency. We show that genotoxin-enhanced Rad1 sumoylation occurs after the nuclease is recruited to lesion sites. A single lysine outside Rad1's nuclease and Rad10-binding domains is sumoylated in vivo and in vitro. Mutation of this site to arginine abolishes Rad1 sumoylation and impairs Rad1-mediated repair at high doses of DNA damage, but sustains the repair of a single double-stranded break. The timing of Rad1 sumoylation and the phenotype bias toward high lesion loads point to a post-incision role for sumoylation, possibly affecting Rad1 dissociation from DNA.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleic Acids Research

ISSN

0305-1048

e-ISSN

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Volume of the periodical

42

Issue of the periodical within the volume

10

Country of publishing house

GB - UNITED KINGDOM

Number of pages

12

Pages from-to

6393-6404

UT code for WoS article

000338768100030

EID of the result in the Scopus database

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