Sumoylation regulates the stability and nuclease activity of Saccharomyces cerevisiae Dna2
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F19%3A00108020" target="_blank" >RIV/00216224:14110/19:00108020 - isvavai.cz</a>
Alternative codes found
RIV/00159816:_____/19:00071035
Result on the web
<a href="http://dx.doi.org/10.1038/s42003-019-0428-0" target="_blank" >http://dx.doi.org/10.1038/s42003-019-0428-0</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s42003-019-0428-0" target="_blank" >10.1038/s42003-019-0428-0</a>
Alternative languages
Result language
angličtina
Original language name
Sumoylation regulates the stability and nuclease activity of Saccharomyces cerevisiae Dna2
Original language description
Dna2 is an essential nuclease-helicase that acts in several distinct DNA metabolic pathways including DNA replication and recombination. To balance these functions and prevent unscheduled DNA degradation, Dna2 activities must be regulated. Here we show that Saccharomyces cerevisiae Dna2 function is controlled by sumoylation. We map the sumoylation sites to the N-terminal regulatory domain of Dna2 and show that in vitro sumoylation of recombinant Dna2 impairs its nuclease but not helicase activity. In cells, the total levels of the non-sumoylatable Dna2 variant are elevated. However, non-sumoylatable Dna2 shows impaired nuclear localization and reduced recruitment to foci upon DNA damage. Non-sumoylatable Dna2 reduces the rate of DNA end resection, as well as impedes cell growth and cell cycle progression through S phase. Taken together, these findings show that in addition to Dna2 phosphorylation described previously, Dna2 sumoylation is required for the homeostasis of the Dna2 protein function to promote genome stability.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10608 - Biochemistry and molecular biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2019
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
COMMUNICATIONS BIOLOGY
ISSN
2399-3642
e-ISSN
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Volume of the periodical
2
Issue of the periodical within the volume
MAY 8 2019
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
1-12
UT code for WoS article
000467215700004
EID of the result in the Scopus database
2-s2.0-85071051748