Sumoylation regulates EXO1 stability and processing of DNA damage

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F15%3A00081061" target="_blank" >RIV/00216224:14110/15:00081061 - isvavai.cz</a>

Alternative codes found

RIV/00159816:_____/15:00063107

Result on the web

<a href="http://dx.doi.org/10.1080/15384101.2015.1060381" target="_blank" >http://dx.doi.org/10.1080/15384101.2015.1060381</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/15384101.2015.1060381" target="_blank" >10.1080/15384101.2015.1060381</a>

Result language

angličtina

Original language name

Sumoylation regulates EXO1 stability and processing of DNA damage

Original language description

DNA double-strand break repair by the error-free pathway of homologous recombination (HR) requires the concerted action of several factors. Among these, EXO1 and DNA2/BLM are responsible for the extensive resection of DNA ends to produce 3'-overhangs, which are essential intermediates for downstream steps of HR. Here we show that EXO1 is a SUMO target and that sumoylation affects EXO1 ubiquitylation and protein stability. We identify an UBC9-PIAS1/PIAS4-dependent mechanism controlling human EXO1 sumoylation in vivo and demonstrate conservation of this mechanism in yeast by the Ubc9-Siz1/Siz2 using an in vitro reconstituted system. Furthermore, we show physical interaction between EXO1 and the de-sumoylating enzyme SENP6 both in vitro and in vivo, promoting EXO1 stability. Finally, we identify the major sites of sumoylation in EXO1 and show that ectopic expression of a sumoylation-deficient form of EXO1 rescues the DNA damage-induced chromosomal aberrations observed upon wt-EXO1 express

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Cell Cycle

ISSN

1538-4101

e-ISSN

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Volume of the periodical

14

Issue of the periodical within the volume

15

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

2439-2450

UT code for WoS article

000359768900015

EID of the result in the Scopus database

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