SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F16%3A00065028" target="_blank" >RIV/00159816:_____/16:00065028 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14110/16:00088247

Result on the web

<a href="http://dx.doi.org/10.1016/j.dnarep.2016.04.001" target="_blank" >http://dx.doi.org/10.1016/j.dnarep.2016.04.001</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.dnarep.2016.04.001" target="_blank" >10.1016/j.dnarep.2016.04.001</a>

Result language

angličtina

Original language name

SUMOylation of Rad52-Rad59 synergistically change the outcome of mitotic recombination

Original language description

Homologous recombination (HR) is essential for maintenance of genome stability through double-strand break (DSB) repair, but at the same time HR can lead to loss of heterozygosity and uncontrolled recombination can be genotoxic. The post-translational modification by SUMO (small ubiquitin-like modifier) has been shown to modulate recombination, but the exact mechanism of this regulation remains unclear. Here we show that SUMOylation stabilizes the interaction between the recombination mediator Rad52 and its paralogue Rad59 in Saccharomyces cerevisiae. Although Rad59 SUMOylation is not required for survival after genotoxic stress, it affects the outcome of recombination to promote conservative DNA repair. In some genetic assays, Rad52 and Rad59 SUMOylation act synergistically. Collectively, our data indicate that the described SUMO modifications affect the balance between conservative and non-conservative mechanisms of HR.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

DNA Repair

ISSN

1568-7864

e-ISSN

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Volume of the periodical

42

Issue of the periodical within the volume

JUN

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

15

Pages from-to

11-25

UT code for WoS article

000377727400002

EID of the result in the Scopus database

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