Atomic force microscopy combined with human pluripotent stem cell derived cardiomyocytes for biomechanical sensing
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F16%3A00065292" target="_blank" >RIV/00159816:_____/16:00065292 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14110/16:00087965
Result on the web
<a href="http://dx.doi.org/10.1016/j.bios.2016.05.073" target="_blank" >http://dx.doi.org/10.1016/j.bios.2016.05.073</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.bios.2016.05.073" target="_blank" >10.1016/j.bios.2016.05.073</a>
Alternative languages
Result language
angličtina
Original language name
Atomic force microscopy combined with human pluripotent stem cell derived cardiomyocytes for biomechanical sensing
Original language description
Cardiomyocyte contraction and relaxation are important parameters of cardiac function altered in many heart pathologies. Biosensing of these parameters represents an important tool in drug development and disease modeling. Human embryonic stem cells and especially patient specific induced pluripotent stem cell-derived cardiomyocytes are well established as cardiac disease model.. Here, a live stem cell derived embryoid body (EB) based cardiac cell syncytium served as a biorecognition element coupled to the microcantilever probe from atomic force microscope thus providing reliable micromechanical cellular biosensor suitable for whole-day testing. The biosensor was optimized regarding the type of cantilever, temperature and exchange of media; in combination with standardized protocol, it allowed testing of compounds and conditions affecting the biomechanical properties of EB. The studied effectors included calcium , drugs modulating the catecholaminergic fight-or-flight stress response such as the beta-adrenergic blocker metoprolol and the beta-adrenergic agonist isoproterenol. Arrhythmogenic effects were studied using caffeine. Furthermore, with EBs originating from patient's stem cells, this biosensor can help to characterize heart diseases such as dystrophies.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
BO - Biophysics
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Biosensors & Bioelectronics
ISSN
0956-5663
e-ISSN
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Volume of the periodical
85
Issue of the periodical within the volume
NOV
Country of publishing house
GB - UNITED KINGDOM
Number of pages
7
Pages from-to
751-757
UT code for WoS article
000382410100100
EID of the result in the Scopus database
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