Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F17%3A00067142" target="_blank" >RIV/00159816:_____/17:00067142 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26620/17:PU123929

Result on the web

<a href="http://dx.doi.org/10.1007/978-1-4939-7021-6_30" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-7021-6_30</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1007/978-1-4939-7021-6_30" target="_blank" >10.1007/978-1-4939-7021-6_30</a>

Result language

angličtina

Original language name

Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy

Original language description

Cell culture methods have been developed in efforts to produce biologically relevant systems for developmental and disease modeling, and appropriate analytical tools are essential. Knowledge of ultrastructural characteristics represents the basis to reveal in situ the cellular morphology, cell-cell interactions, organelle distribution, niches in which cells reside, and many more. The traditional method for 3D visualization of ultrastructural components, serial sectioning using transmission electron microscopy (TEM), is very labor-intensive due to contentious TEM slice preparation and subsequent image processing of the whole collection. In this chapter, we present serial block-face scanning electron microscopy, together with complex methodology for spheroid formation, contrasting of cellular compartments, image processing, and 3D visualization. The described technique is effective for detailed morphological analysis of stem cell spheroids, organoids, as well as organotypic cell cultures.

Czech name

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Czech description

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Type

C - Chapter in a specialist book

CEP classification

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OECD FORD branch

10601 - Cell biology

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Book/collection name

3D Cell Culture

ISBN

978-1-4939-7019-3

Number of pages of the result

15

Pages from-to

1-15

Number of pages of the book

452

Publisher name

Humana Press

Place of publication

USA

UT code for WoS chapter

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