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Revealing 3D ultrastructure and morphology of stem cell spheroids by electron microscopy

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216305%3A26620%2F17%3APU143838" target="_blank" >RIV/00216305:26620/17:PU143838 - isvavai.cz</a>

  • Result on the web

    <a href="https://www.scopus.com/record/display.uri?eid=2-s2.0-85021355973&origin=resultslist&sort=plf-f&src=s&st1=Revealing+3D+ultrastructure+and+morphology+of+stem+cell+spheroids+by+electron+microscopy&sid=0976ed42b559776de22e21e63e60e432&sot=b&sdt=b&sl=103&s=TI" target="_blank" >https://www.scopus.com/record/display.uri?eid=2-s2.0-85021355973&origin=resultslist&sort=plf-f&src=s&st1=Revealing+3D+ultrastructure+and+morphology+of+stem+cell+spheroids+by+electron+microscopy&sid=0976ed42b559776de22e21e63e60e432&sot=b&sdt=b&sl=103&s=TI</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/978-1-4939-7021-6_30" target="_blank" >10.1007/978-1-4939-7021-6_30</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Revealing 3D ultrastructure and morphology of stem cell spheroids by electron microscopy

  • Original language description

    Cell culture methods have been developed in efforts to produce biologically relevant systems for developmental and disease modeling, and appropriate analytical tools are essential. Knowledge of ultrastructural characteristics represents the basis to reveal in situ the cellular morphology, cell-cell interactions, organelle distribution, niches in which cells reside, and many more. The traditional method for 3D visualization of ultrastructural components, serial sectioning using transmission electron microscopy (TEM), is very labor-intensive due to contentious TEM slice preparation and subsequent image processing of the whole collection. In this chapter, we present serial block-face scanning electron microscopy, together with complex methodology for spheroid formation, contrasting of cellular compartments, image processing, and 3D visualization. The described technique is effective for detailed morphological analysis of stem cell spheroids, organoids, as well as organotypic cell cultures.

  • Czech name

  • Czech description

Classification

  • Type

    J<sub>SC</sub> - Article in a specialist periodical, which is included in the SCOPUS database

  • CEP classification

  • OECD FORD branch

    10608 - Biochemistry and molecular biology

Result continuities

  • Project

    <a href="/en/project/LQ1601" target="_blank" >LQ1601: CEITEC 2020</a><br>

  • Continuities

    P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Others

  • Publication year

    2017

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Name of the periodical

    Methods in Molecular Biology

  • ISSN

    1940-6029

  • e-ISSN

  • Volume of the periodical

    1612

  • Issue of the periodical within the volume

    1

  • Country of publishing house

    US - UNITED STATES

  • Number of pages

    15

  • Pages from-to

    417-431

  • UT code for WoS article

  • EID of the result in the Scopus database

    2-s2.0-85021355973