Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14110%2F17%3A00095166" target="_blank" >RIV/00216224:14110/17:00095166 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/978-1-4939-7021-6_30" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-7021-6_30</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-4939-7021-6_30" target="_blank" >10.1007/978-1-4939-7021-6_30</a>
Alternative languages
Result language
angličtina
Original language name
Revealing 3D Ultrastructure and Morphology of Stem Cell Spheroids by Electron Microscopy
Original language description
Cell culture methods have been developed in efforts to produce biologically relevant systems for developmental and disease modeling, and appropriate analytical tools are essential. Knowledge of ultrastructural characteristics represents the basis to reveal in situ the cellular morphology, cell-cell interactions, organelle distribution, niches in which cells reside, and many more. The traditional method for 3D visualization of ultrastructural components, serial sectioning using transmission electron microscopy (TEM), is very labor-intensive due to contentious TEM slice preparation and subsequent image processing of the whole collection. In this chapter, we present serial block-face scanning electron microscopy, together with complex methodology for spheroid formation, contrasting of cellular compartments, image processing, and 3D visualization. The described technique is effective for detailed morphological analysis of stem cell spheroids, organoids, as well as organotypic cell cultures
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
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OECD FORD branch
10600 - Biological sciences
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
3D Cell Culture : Methods and Protocols
ISBN
9781493970193
ISSN
1064-3745
e-ISSN
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Number of pages
15
Pages from-to
417-431
Publisher name
Humana Press
Place of publication
New York
Event location
New York
Event date
Jan 1, 2017
Type of event by nationality
CST - Celostátní akce
UT code for WoS article
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