Transcriptional profiling of murine osteoblast differentiation based on RNA-seq expression analyses

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00159816%3A_____%2F18%3A00069357" target="_blank" >RIV/00159816:_____/18:00069357 - isvavai.cz</a>

Alternative codes found

RIV/00216305:26220/18:PU127667

Result on the web

<a href="http://dx.doi.org/10.1016/j.bone.2018.04.006" target="_blank" >http://dx.doi.org/10.1016/j.bone.2018.04.006</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.bone.2018.04.006" target="_blank" >10.1016/j.bone.2018.04.006</a>

Result language

angličtina

Original language name

Transcriptional profiling of murine osteoblast differentiation based on RNA-seq expression analyses

Original language description

Osteoblastic differentiation is a multistep process characterized by osteogenic induction of mesenchymal stem cells, which then differentiate into proliferative pre-osteoblasts that produce copious amounts of extracellular matrix, followed by stiffening of the extracellular matrix, and matrix mineralization by hydroxylapatite deposition. Although these processes have been well characterized biologically, a detailed transcriptional analysis of murine primary calvaria osteoblast differentiation based on RNA sequencing (RNA-seq) analyses has not previously been reported. Here, we used RNA-seq to obtain expression values of 29,148 genes at four time points as murine primary calvaria osteoblasts differentiate in vitro until onset of mineralization was clearly detectable by microscopic inspection. Expression of marker genes confirmed osteogenic differentiation. We explored differential expression of 1386 protein-coding genes using unsupervised clustering and GO analyses. 100 differentially expressed lncRNAs were investigated by co-expression with protein-coding genes that are localized within the same topologically associated domain. Additionally, we monitored expression of 237 genes that are silent or active at distinct time points and compared differential exon usage. Our data represent an in-depth profiling of murine primary calvaria osteoblast differentiation by RNA-seq and contribute to our understanding of genetic regulation of this key process in osteoblast biology.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30202 - Endocrinology and metabolism (including diabetes, hormones)

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Bone

ISSN

8756-3282

e-ISSN

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Volume of the periodical

113

Issue of the periodical within the volume

August

Country of publishing house

US - UNITED STATES

Number of pages

12

Pages from-to

29-40

UT code for WoS article

000437550800004

EID of the result in the Scopus database

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