Osteogenic Potential of the Transcription Factor c-MYB
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F67985904%3A_____%2F17%3A00474406" target="_blank" >RIV/67985904:_____/17:00474406 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/17:00094639 RIV/00159816:_____/17:00066960 RIV/62157124:16170/17:43874454
Result on the web
<a href="http://dx.doi.org/10.1007/s00223-016-0219-2" target="_blank" >http://dx.doi.org/10.1007/s00223-016-0219-2</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/s00223-016-0219-2" target="_blank" >10.1007/s00223-016-0219-2</a>
Alternative languages
Result language
angličtina
Original language name
Osteogenic Potential of the Transcription Factor c-MYB
Original language description
The transcription factor c-MYB is a well-known marker of undifferentiated cells such as haematopoietic cell precursors, but recently it has also been observed in differentiated cells that produce hard tissues. Our previous findings showed the presence of c-MYB in intramembranous bones and its involvement in the chondrogenic steps of endochondral ossification, where the up-regulation of early chondrogenic markers after c-myb overexpression was observed. Since we previously detected c-MYB in osteoblasts, we aimed to analyse the localisation of c-MYB during later stages of endochondral bone formation and address its function during bone matrix production. c-MYB-positive cells were found in the chondro-osseous junction zone in osteoblasts of trabecular bone as well as deeper in the zone of ossification in cells of spongy bone. To experimentally evaluate the osteogenic potential of c-MYB during endochondral bone formation, micromasses derived from embryonic mouse limb buds were established. Nuclear c-MYB protein expression was observed in long-term micromasses, especially in the areas around nodules. c-myb overexpression induced the expression of osteogenic-related genes such as Bmp2, Comp, Csf2 and Itgb1. Moreover, alizarin red staining and osteocalcin labelling promoted mineralised matrix production in c-myb-overexpressing cultures, whereas downregulation of c-myb by siRNA reduced mineralised matrix production. In conclusion, c-Myb plays a role in the osteogenesis of long bones by inducing osteogenic genes and causing the enhancement of mineral matrix production. This action of the transcription factor c-Myb might be of interest in the future for the establishment of novel approaches to tissue regeneration.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10605 - Developmental biology
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2017
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Calcified Tissue International and Calcified Tissue Research
ISSN
0171-967X
e-ISSN
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Volume of the periodical
100
Issue of the periodical within the volume
3
Country of publishing house
US - UNITED STATES
Number of pages
12
Pages from-to
311-322
UT code for WoS article
000395129400011
EID of the result in the Scopus database
2-s2.0-85007242104