Dental pulp stem cells and their characterization

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F09%3A00201755" target="_blank" >RIV/00179906:_____/09:00201755 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11150/09:00208772

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Dental pulp stem cells and their characterization

Original language description

We isolated and expanded 16 lines of DPSCs in three different media over 40 population doublings. DPSCs cultivated in all media were cytogenetically stable and showed no signs of spontaneous differentiation. Over the entire cultivation period, we observed no changes in cell viability. Medium containing 2 % FCS supplemented with ITS provided better cultivation conditions for DPSCs (shorter DT and more stable proliferation activity were measured) than other tested media (e.g. most cited 10 % FCS or high FCS concentration media). Decrease in concentration of FCS and adding ITS into media had no negative effects on basics biological characteristics(viability, cell diameter). DPSCs cultivated in 2 % FCS with ITS showed lower positivity mesenchymal stem cell markers and HLA I compared to DPSCs cultivated in 2 % FCS or 10 % FCS media.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30106 - Anatomy and morphology (plant science to be 1.6)

Project

<a href="/en/project/NR9182" target="_blank" >NR9182: Isolation, cultivation, differentiation and phenotypic characterization of dental pulp stem cells</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Biomedical Papers

ISSN

1213-8118

e-ISSN

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Volume of the periodical

153

Issue of the periodical within the volume

1

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

6

Pages from-to

31-36

UT code for WoS article

000265639300006

EID of the result in the Scopus database

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