Human Plasma and Human Platelet-rich Plasma as a Substitute for Fetal Calf Serum during Long-term Cultivation of Mesenchymal Dental Pulp Stem Cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F14%3A10283985" target="_blank" >RIV/00179906:_____/14:10283985 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11150/14:10283985

Result on the web

<a href="http://orbis.lfhk.cuni.cz/Acta_Medica/2014/2014_119.pdf" target="_blank" >http://orbis.lfhk.cuni.cz/Acta_Medica/2014/2014_119.pdf</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.14712/18059694.2014.50" target="_blank" >10.14712/18059694.2014.50</a>

Result language

angličtina

Original language name

Human Plasma and Human Platelet-rich Plasma as a Substitute for Fetal Calf Serum during Long-term Cultivation of Mesenchymal Dental Pulp Stem Cells

Original language description

Our aims were to isolate and cultivate mesenchymal dental pulp stem cells (DPSC) in various media enriched with human blood derivatives, and subsequently to investigate their basic biological properties. Methods: DPSC were cultivated in five different media based on ? MEM containing different concentrations of human plasma (HP), platelet-rich plasma (PRP), or fetal calf serum (FCS). The DPSC biological properties were examined periodically. Results: We cultivated DPSC in the various cultivation media over 15 population doublings except for the medium supplemented with 10% HP. Our results showed that DPSC cultivated in medium supplemented with 10% PRP showed the shortest average population doubling time (DT) (28.6 +- 4.6 hours), in contrast to DPSC cultivated in 10% HP which indicated the longest DT (156.2 +- 17.8 hours); hence this part of the experiment had been cancelled in the 6th passage. DPSC cultivated in media with 2% FCS+ITS (DT 47.3 +- 10.4 hours), 2% PRP (DT 40.1 +- 5.7 hours

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FF - ENT (ie. ear, nose, throat), ophthalmology, dentistry

OECD FORD branch

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Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2014

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Medica (Hradec Králové)

ISSN

1211-4286

e-ISSN

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Volume of the periodical

57

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

119-126

UT code for WoS article

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EID of the result in the Scopus database

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