The effect of fetal calf serum on human dental pulp stem cells

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11150%2F13%3A10190661" target="_blank" >RIV/00216208:11150/13:10190661 - isvavai.cz</a>

Result on the web

<a href="http://orbis.lfhk.cuni.cz/Acta_Medica/2013/2013_142.pdf" target="_blank" >http://orbis.lfhk.cuni.cz/Acta_Medica/2013/2013_142.pdf</a>

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

The effect of fetal calf serum on human dental pulp stem cells

Original language description

Aims: Authors studied potential side effects of fetal calf serum (FCS) in cultivation media on human dental pulp stem cells (DPSC) during long term cultivation. Methods: Two lines of DPSC obtained healthy donors (male 22 years, female 23 years) were used. Both lines were cultivated under standard cultivation conditions in four different media containing 10% or 2% FCS and substituted with growth factors. During long term cultivation proliferation ability, karyotype and phenotype of DPSC were measured. Results: Both lines of DPSC cultivated in a media containing 2% FCS and ITS supplement showed the highest number of population doublings. On the other hand the proliferation rate of DPSC cultivated in a media with 2%FCS without ITS supplement was slowest.Proliferation rate of DPSC cultivated in 10% FCS media with or without FGF-2 was comparable. DPSC cultivated in a media with 10% FCS showed a significantly higher amount of chromosomal aberrations. These chromosomal aberrations do not see

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FF - ENT (ie. ear, nose, throat), ophthalmology, dentistry

OECD FORD branch

—

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2013

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Acta Medica (Hradec Králové)

ISSN

1211-4286

e-ISSN

—

Volume of the periodical

56

Issue of the periodical within the volume

4

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

8

Pages from-to

142-149

UT code for WoS article

—

EID of the result in the Scopus database

—