Effective, convenient, and green sample preparation for the determination of retinol and retinol acetate in human serum using pipette tip microextraction

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00179906%3A_____%2F23%3A10465842" target="_blank" >RIV/00179906:_____/23:10465842 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11160/23:10465842

Result on the web

<a href="https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=0WXe-dmg1" target="_blank" >https://verso.is.cuni.cz/pub/verso.fpl?fname=obd_publikace_handle&handle=0WXe-dmg1</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.talanta.2023.124689" target="_blank" >10.1016/j.talanta.2023.124689</a>

Result language

angličtina

Original language name

Effective, convenient, and green sample preparation for the determination of retinol and retinol acetate in human serum using pipette tip microextraction

Original language description

An efficient sample preparation based on pipette tip microextraction that can be used for the analysis of retinol in human serum has been developed. Altogether, nine commercial pipette tips were compared based on recovery, sample volume, use of organic solvent, handling difficulty, duration of the preparation process, price, and greenness of the method. Retinol acetate was used as the internal standard. The extraction efficiency for both compounds was evaluated to optimize and select the best pipette tip for sample preparation, which was the WAX-S XTR pipette tip containing an ion exchanger and salt. This tip combined solid phase extraction and salting-out assisted liquid-liquid extraction. Satisfying recoveries of 100 and 80% for retinol and retinol acetate, respec-tively, and good repeatability were demonstrated. The action of this pipette tip was based on the clean-up workflow in which the interferences were retained on the sorbent. The presence of residual interferences in the extracted samples did not affect the HPLC separation of compounds of interest. The simplicity of the clean-up workflow reduced the time of the sample preparation compared to the bind-wash-elute counterpart workflow. The advantages of our technique are its environmental friendliness and cost effectiveness. The selected pipette tip with an excellent microextraction efficiency enables sample preparation in both clinical research and practice.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30104 - Pharmacology and pharmacy

Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2023

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Talanta

ISSN

0039-9140

e-ISSN

1873-3573

Volume of the periodical

262

Issue of the periodical within the volume

SEP

Country of publishing house

NL - THE KINGDOM OF THE NETHERLANDS

Number of pages

9

Pages from-to

124689

UT code for WoS article

001007484800001

EID of the result in the Scopus database

2-s2.0-85159554456