Complex analysis of the p53 tumor suppressor in lung carcinoma

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209775%3A_____%2F16%3AN0000021" target="_blank" >RIV/00209775:_____/16:N0000021 - isvavai.cz</a>

Alternative codes found

RIV/00216224:14310/16:00089204 RIV/65269705:_____/16:00064344

Result on the web

<a href="https://www.spandidos-publications.com/or/35/3" target="_blank" >https://www.spandidos-publications.com/or/35/3</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.3892/or.2015.4533" target="_blank" >10.3892/or.2015.4533</a>

Result language

angličtina

Original language name

Complex analysis of the p53 tumor suppressor in lung carcinoma

Original language description

Lung cancer is the leading cause of cancer-related deaths worldwide. The p53 tumor suppressor is a transcription factor controlling expression of its target genes in response to various stress stimuli. Mutations of the TP53 gene occur very frequently in lung carcinomas and they play an important role in both oncogenic transformation of lung epithelial cells and lung carcinoma progression. We determined the TP53 status in 42 samples of squamous cell lung carcinoma (SQCC) and 56 samples of lung adenocarcinoma (AC) by the functional analysis FASAY and its variant called split assay. Altogether, we detected 64 TP53 mutations in 63 patients and analyzed them by cDNA and gDNA sequencing. The TP53 mutations were found in 76.2% (32/42) of SQCC cases, and 55.4% (31/56) of ACs. Immunoblotting revealed the p53 protein accumulation in 18 samples (42.9%) among SQCC cases and 19 samples (33.9%) among AC cases. Using fluorescence in situ hybridization we detected loss of the TP53-specific 17p13.3 locus in 23 from 41 analyzed SQCC samples (56.1%) and in 20 from 54 analyzed AC samples (37.0%). We did not find any statistically significant differences in overall and disease-free survival in relation to TP53 status.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

—

Project

<a href="/en/project/NT13784" target="_blank" >NT13784: Complex analysis of p53 mutants in lymphoproliferative diseases</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2016

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Oncology reports

ISSN

1021-335X

e-ISSN

—

Volume of the periodical

35

Issue of the periodical within the volume

3

Country of publishing house

GR - GREECE

Number of pages

9

Pages from-to

1859-1867

UT code for WoS article

—

EID of the result in the Scopus database

—