Supercoil-Selective binding of protein p53: influence of the protein redox state and roles of p53 domains

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F00%3A00000102" target="_blank" >RIV/00209805:_____/00:00000102 - isvavai.cz</a>

Result on the web

—

DOI - Digital Object Identifier

—

Result language

angličtina

Original language name

Supercoil-Selective binding of protein p53: influence of the protein redox state and roles of p53 domains

Original language description

Full length wild type tumor suppressor protein p53 preferentially binds to supercoiled DNA regardless of the presence or absence of the p53 consensus sequence. This supercoil-selective binding was partially inhibited by the protein oxidation by diamide and p53 sequence-specific DNA binding was completely abolished. The roles of the two protein domains in the SCS binding of reduced (p53red) and oxidized (p53ox) p53 were studied using truncated p53 molecules and monoclonal antibodies (MAb). The primary site responsible for the strong SCS p53 DNA binding is the protein C-terminus. Those p53 constructs lacking free C-terminus in the reduced state bind sc and linear DNAs with similar affinities.

Czech name

—

Czech description

—

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FD - Oncology and haematology

OECD FORD branch

—

Project

—

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2000

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Journal of Biomolecular Structure and Dynamics

ISSN

0739-1102

e-ISSN

—

Volume of the periodical

17

Issue of the periodical within the volume

6

Country of publishing house

US - UNITED STATES

Number of pages

6

Pages from-to

—

UT code for WoS article

—

EID of the result in the Scopus database

—