Transgelin is upregulated in stromal cell sof lymf node positive breast cancer
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F16%3AN0000008" target="_blank" >RIV/00209805:_____/16:N0000008 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.jprot.2015.11.025" target="_blank" >http://dx.doi.org/10.1016/j.jprot.2015.11.025</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jprot.2015.11.025" target="_blank" >10.1016/j.jprot.2015.11.025</a>
Alternative languages
Result language
angličtina
Original language name
Transgelin is upregulated in stromal cell sof lymf node positive breast cancer
Original language description
Transgelin and transgelin-2 have been discussed as potential markers of various cancers. Here we identified increased transgelin level in lymph node positive vs. negative, low grade primary breast cancer tissues using 2-DE in the cohort of 12 patients.Wefurther clinically validated 2-DE results in an independent cohort of 48 lowgrade breast cancer patients through untargeted and targeted proteomics analysis (iTRAQ-2D-LC–MS/MS, mTRAQSRM), at transcript level and using immunohistochemistry. Another group of 48 high grade tumors of different breast cancer subtypes was analyzed together with the low grade samples to test transgelin specificity for low grade tumors and to study transgelin relation to known molecular markers and histological features. The results confirmed transgelin connection with the lymph node metastasis. As a marker of a reactive tumor stroma, transgelin can be connected with the higher risk of metastasis development. Moreover, we observed signifiant down-regulation of transgelin in high vs. low grade tumors caused by decreased content of stromal cells (mainly expressing transgelin) in high grade tumor tissue. We also analyzed expression of transgelin-2 in the sekond cohort using proteomics and immunohistochemistry. Transgelin-2 was mainly expressed by epithelial cancer cells and its levels were increased in metastatic and poorly differentiated tumors. Biological significance: Both transgelin and transgelin-2 have been previously described as potential markers of many types of cancer.Weare specifying this connection to metastatic affection of lymph nodes and cell differentiation in breast cancer. In thewider context, the results of our study highlight tumor stromaas a source of cancer biomarkers and point out how measured levels of tissue markers can actually reflect cellular feature of cancer mass.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Journal of Proteomics
ISSN
1874-3919
e-ISSN
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Volume of the periodical
132
Issue of the periodical within the volume
30 January 2016
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
9
Pages from-to
103-111
UT code for WoS article
000368747400011
EID of the result in the Scopus database
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