DNA Methylation in Solid Tumors: Functions and Methods of Detection

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F21%3A00078599" target="_blank" >RIV/00209805:_____/21:00078599 - isvavai.cz</a>
Result on the web
<a href="https://www.mdpi.com/1422-0067/22/8/4247" target="_blank" >https://www.mdpi.com/1422-0067/22/8/4247</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.3390/ijms22084247" target="_blank" >10.3390/ijms22084247</a>

Result language
angličtina
Original language name
DNA Methylation in Solid Tumors: Functions and Methods of Detection
Original language description
DNA methylation, i.e., addition of methyl group to 5'-carbon of cytosine residues in CpG dinucleotides, is an important epigenetic modification regulating gene expression, and thus implied in many cellular processes. Deregulation of DNA methylation is strongly associated with onset of various diseases, including cancer. Here, we review how DNA methylation affects carcinogenesis process and give examples of solid tumors where aberrant DNA methylation is often present. We explain principles of methods developed for DNA methylation analysis at both single gene and whole genome level, based on (i) sodium bisulfite conversion, (ii) methylation-sensitive restriction enzymes, and (iii) interactions of 5-methylcytosine (5mC) with methyl-binding proteins or antibodies against 5mC. In addition to standard methods, we describe recent advances in next generation sequencing technologies applied to DNA methylation analysis, as well as in development of biosensors that represent their cheaper and faster alternatives. Most importantly, we highlight not only advantages, but also disadvantages and challenges of each method
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10608 - Biochemistry and molecular biology

Project
<a href="/en/project/GA19-02014S" target="_blank" >GA19-02014S: Identification of determinants associated with metastatic colorectal cancer in relation to Anterior gradient 2</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2021
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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