TAp73 and ΔTAp73 isoforms show cell-type specific distributions and alterations in cancer
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00209805%3A_____%2F24%3A00080060" target="_blank" >RIV/00209805:_____/24:00080060 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14310/24:00138952
Result on the web
<a href="https://pubmed.ncbi.nlm.nih.gov/39622910/" target="_blank" >https://pubmed.ncbi.nlm.nih.gov/39622910/</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1038/s41598-024-80927-9" target="_blank" >10.1038/s41598-024-80927-9</a>
Alternative languages
Result language
angličtina
Original language name
TAp73 and ΔTAp73 isoforms show cell-type specific distributions and alterations in cancer
Original language description
TP73 is a member of the TP53 gene family and produces N- and C-terminal protein isoforms through alternative promoters, alternative translation initiation and alternative splicing. Most notably, p73 protein isoforms may either contain a p53-like transactivation domain (TAp73 isoforms) or lack this domain (ΔTAp73 isoforms) and these variants have opposing or independent functions. To date, there is a lack of well-characterised isoform-specific p73 antibodies. Here, we produced polyclonal and monoclonal antibodies to N-terminal p73 variants and the C-terminal p73α isoform, the most common variant in human tissues. These reagents show that TAp73 is a marker of multiciliated epithelial cells, while ΔTAp73 is a marker of non-proliferative basal/reserve cells in squamous epithelium. We were unable to detect ΔNp73 variant proteins, in keeping with recent data that this is a minor form in human tissues. Most cervical squamous cell carcinomas (79%) express p73α, and the distribution of staining in basal cells correlated with lower tumour grade. TAp73 was found in 17% of these tumours, with a random distribution and no association with clinicopathological features. These data indicate roles for ΔTAp73 in maintaining a non-proliferative state of undifferentiated squamous epithelial cells and for TAp73 in the production of differentiated multiciliated cells.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30204 - Oncology
Result continuities
Project
<a href="/en/project/GA23-05951S" target="_blank" >GA23-05951S: Mechanisms and Effects of Inverse TAp63 and ΔNp63 Regulation</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2024
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Scientific reports
ISSN
2045-2322
e-ISSN
2045-2322
Volume of the periodical
14
Issue of the periodical within the volume
1
Country of publishing house
GB - UNITED KINGDOM
Number of pages
16
Pages from-to
29949
UT code for WoS article
001376887400020
EID of the result in the Scopus database
2-s2.0-85211162307