Cytokeratins 8 and 18 in adult human corneal endothelium

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F09%3A3500" target="_blank" >RIV/00216208:11110/09:3500 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11120/09:00001715 RIV/00064165:_____/09:3500

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Cytokeratins 8 and 18 in adult human corneal endothelium

Original language description

The aim of this study was to determine if cytokeratins (CKs) 8 and 18 - typical epithelial cell markers - are expressed in human corneal endothelium. Cryosections, paraffin-embedded sections and corneal endothelial imprints obtained from eleven corneal discs were used. Different fixative solutions were applied before indirect immunofluorescent or enzymatic staining. Antibodies against CK8, CK18 and CK8/18 were used and semi-quantitative RT-PCR and Western blotting were performed. Approximately 50% of the corneal endothelial cells were positive for CK8, CK18 and the CK8/18 after acetone fixation. No signal was detected when 4% formalin or 10% paraformaldehyde was used. CK8 and CK18 were detected in the endothelium of all tested corneas by Western blotting or semi-quantitative RT-PCR, respectively. We detected CK8 and CK18 in the endothelium of all specimens. These results clearly demonstrate that cells of the corneal endothelium express CKs 8 and 18 and share some features with simple e

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FF - ENT (ie. ear, nose, throat), ophthalmology, dentistry

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2009

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Experimental Eye Research

ISSN

0014-4835

e-ISSN

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Volume of the periodical

89

Issue of the periodical within the volume

3

Country of publishing house

US - UNITED STATES

Number of pages

6

Pages from-to

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UT code for WoS article

000269041900016

EID of the result in the Scopus database

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