Apoptosis and necrosis in vaccinia virus-infected He La G and BSC-40 cells
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F11%3A8927" target="_blank" >RIV/00216208:11110/11:8927 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1016/j.virusres.2011.05.005" target="_blank" >http://dx.doi.org/10.1016/j.virusres.2011.05.005</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Apoptosis and necrosis in vaccinia virus-infected He La G and BSC-40 cells
Original language description
In most cells, vaccinia virus (VACV) infection is considered to cause a lytic cell death, an equivalent of necrosis. In this paper, we have further analyzed the type of cell death in VACV-infected cells HeLa G and BSC-40. In a cell-based flow cytometricassay, we showed a specific activation of caspase-2 and 4 in HeLa G and BSC-40 cells infected with VACV, strain WR. The actual activity of the two caspases, but also of caspase-3, was then confirmed in lysates of infected HeLa G, but not in BSC-40 cells.PARP cleavage, as well as morphological features of apoptosis and/or activity of the executioner caspase-3 in situ were found increased only in infected HeLa G cells. Finally, vaccination strains Dryvax and Praha were found to induce apoptosis in both HeLa G and BSC-40 cells, as characterized morphologically and by PARP cleavage. These findings may be important for understanding the differences in VACV-host interactions and post-vaccination complications in different individuals.
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EE - Microbiology, virology
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Virus Research
ISSN
0168-1702
e-ISSN
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Volume of the periodical
160
Issue of the periodical within the volume
1
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
11
Pages from-to
40-50
UT code for WoS article
000295345600005
EID of the result in the Scopus database
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