Imaging of Toxocara canis larvae labelled by CFSE in BALB/c mice

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F11%3A9060" target="_blank" >RIV/00216208:11110/11:9060 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11120/11:00002937 RIV/00023841:_____/11:#0000065

Result on the web

<a href="http://dx.doi.org/10.1007/s00436-010-2145-y" target="_blank" >http://dx.doi.org/10.1007/s00436-010-2145-y</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Imaging of Toxocara canis larvae labelled by CFSE in BALB/c mice

Original language description

Mice are used most often as a model for human toxocarosis caused by Toxocara canis larvae. Variety of symptoms developing during the infection reflects behaviour of the larvae, which are able to escape from the intestine and further invade and damage various host organs. In order to find an approach enabling observation on parasite behaviour in mouse in vivo, we used an epifluorescence method and a small animal imaging system (SAIS). Larvae of T. canis were labelled by carboxyfluorescein succinimidyl ester (CFSE) which incorporated on the parasite gastrointestinal tract. Following infection of BALB/c mice by CFSE-labelled larvae it has been observed that staining had no influence on viability and further migratory activity of the parasites through thehost organs (the intestine, liver, lungs and brain) where they were detected by SAIS until day 17 p.i. In addition, the dye did not affect larval antigenic activity as well as the development of related immune response. Imaging of parasit

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

FN - Epidemiology, infection diseases and clinical immunology

OECD FORD branch

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Project

<a href="/en/project/GA310%2F08%2F1624" target="_blank" >GA310/08/1624: The study on Toxocara migration to the CNS in vivo</a><br>

Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Parasitology Research

ISSN

0932-0113

e-ISSN

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Volume of the periodical

108

Issue of the periodical within the volume

4

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

1007-1014

UT code for WoS article

000288457000030

EID of the result in the Scopus database

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