The evaluation of survival and proliferation of lymphocytes in autologous mixed leukocyte reaction with dendritic cells. The comparison of incorporation of (3)H-thymidine and differential gating method
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F11%3A9189" target="_blank" >RIV/00216208:11110/11:9189 - isvavai.cz</a>
Alternative codes found
RIV/00023736:_____/11:00009277 RIV/00064165:_____/11:9189
Result on the web
<a href="http://dx.doi.org/10.1016/j.cellimm.2011.06.006" target="_blank" >http://dx.doi.org/10.1016/j.cellimm.2011.06.006</a>
DOI - Digital Object Identifier
—
Alternative languages
Result language
angličtina
Original language name
The evaluation of survival and proliferation of lymphocytes in autologous mixed leukocyte reaction with dendritic cells. The comparison of incorporation of (3)H-thymidine and differential gating method
Original language description
Dendritic cells (DCs) play the key role in T-lymphocyte proliferation and induction of antitumour response. The mixed leukocyte reaction (MLR) of T-lymphocytes and DCs is essential instrument for immunological mechanisms studies. Conventionally used method for determination of T-lymphocytes proliferation, 3H-thymidine incorporation, provides only general information. The method of flow cytometry and differential gating, based on time limited acquisition of events and on its distribution according to forward and side scatter values, seems to be more suitable for quantitative and qualitative analysis of T-lymphocyte proliferation. We decided to compare these methods and determine mutual correlation and compatibility. Eleven patients were studied. Both methods retained consistency with regard to survival and proliferation of lymphocytes. However, the correlation was not convincing. Therefore, both methods might be used for evaluation of MLR, but each of them gives specific and complementa
Czech name
—
Czech description
—
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EC - Immunology
OECD FORD branch
—
Result continuities
Project
—
Continuities
Z - Vyzkumny zamer (s odkazem do CEZ)<br>S - Specificky vyzkum na vysokych skolach
Others
Publication year
2011
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Cellular Immunology
ISSN
0008-8749
e-ISSN
—
Volume of the periodical
271
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
78-84
UT code for WoS article
000297658400012
EID of the result in the Scopus database
—