Low antigen-dependent activity of T cells after repeated stimulation using dendritic cells and expansion with interleukin-2
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F65269705%3A_____%2F03%3A00007983" target="_blank" >RIV/65269705:_____/03:00007983 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Low antigen-dependent activity of T cells after repeated stimulation using dendritic cells and expansion with interleukin-2
Original language description
Both CD 8+ and CD 4+ T cells with specific activity against tumor antigens are needed for an efficient antitumor immune response. Activation snd proliferation of T cells require cellular interactions including adhesion,recognition of peptides presented by MHC molecules to the T cells receptor, and costimulation. In a series of experiments we attempted to generate and expand specific T cells by repeated stimulation using antigen-loaded autologous dendritic cells (DCs). Dcs werwobtained from peripheral blood mononuclear cells (PBMC) in the presence of IL ? 4 and GM-CSF. TNF ?á was added to induce maturation. A conjugate of myeloma idiotypic protein wih keyhole limpet hemocyanin was used as antigen. Nonadherent peripheral blood mononuclear cells were cultured in the presence of IL-2 and IL-7. Autologous DCs were added to the lymphocyte on days 3,10, and17. The lymphocytes were stimulated by high concentration of IL-2 between days 21 and 27. Lymphocytes harvested on day 27 proliferated in
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/NC6763" target="_blank" >NC6763: Use of monoclonal immunoglobulin for preparation of antitumor vaccine in patients with multiple myeloma</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2003
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Neoplasma
ISSN
ISSN 0028-268
e-ISSN
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Volume of the periodical
50
Issue of the periodical within the volume
5
Country of publishing house
CZ - CZECH REPUBLIC
Number of pages
4
Pages from-to
120-123
UT code for WoS article
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EID of the result in the Scopus database
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