Glucocerebrosidase gene has an alternative upstream promoter, which has features and expression characteristic of housekeeping genes

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F11%3A9903" target="_blank" >RIV/00216208:11110/11:9903 - isvavai.cz</a>

Alternative codes found

RIV/00023001:_____/11:00002460 RIV/00064165:_____/11:9903

Result on the web

<a href="http://dx.doi.org/10.1016/j.bcmd.2010.12.011" target="_blank" >http://dx.doi.org/10.1016/j.bcmd.2010.12.011</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Glucocerebrosidase gene has an alternative upstream promoter, which has features and expression characteristic of housekeeping genes

Original language description

Database searches have shown that a part of glucocerebrosidase (GBA) transcripts may originate at an alternative upstream promoter (P2) located 2.6 kb upstream of the known (P1) GBA promoter. Luciferase assays confirmed promoter activity of both sites inHepG2 cells: the P1 construct exhibited higher activity of luciferase than the P2. Serial 5 deletions of P2 led to changes in reporter activity. Three P2 transcription initiation sites were found by 5 RACE at positions 347, 380 and 413 bp upstream of the +1 ATG. The expression stability of transcripts from P2, P1 was studied in 20 human tissues and was higher than that of GAPDH and ACTB, which are commonly used as reference housekeeping genes. The P2 contains an unmethylated CpG island, multiple Sp-1 consensus binding sites and, unlike P1, does not contain a TATA box, features all common to the majority of housekeeping gene promoters.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

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Continuities

Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2011

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Blood Cells Molecules and Diseases

ISSN

1079-9796

e-ISSN

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Volume of the periodical

46

Issue of the periodical within the volume

3

Country of publishing house

CZ - CZECH REPUBLIC

Number of pages

7

Pages from-to

239-245

UT code for WoS article

000288574700010

EID of the result in the Scopus database

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