Magnetic Bead Cellulose as a Suitable Support for Immobilization of alpha-Chymotrypsin

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F12%3A11462" target="_blank" >RIV/00216208:11110/12:11462 - isvavai.cz</a>

Alternative codes found

RIV/61389013:_____/12:00382141 RIV/00216208:11310/12:10126758

Result on the web

<a href="http://dx.doi.org/10.1007/s12010-012-9772-y" target="_blank" >http://dx.doi.org/10.1007/s12010-012-9772-y</a>

DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Magnetic Bead Cellulose as a Suitable Support for Immobilization of alpha-Chymotrypsin

Original language description

Magnetic bead cellulose was prepared by a suspension method from the mixture of viscose and magnetite using thermal sol-gel transition and regeneration of cellulose. The prepared magnetic particles after their activation with divinyl sulfone were shown to be suitable magnetic carrier for immobilization of alpha-chymotrypsin and for its application in proteomic studies. The specific activity of the immobilized proteinase was high; its activity did not change in the course of storage. The following properties of the immobilized proteinase were compared with those of the soluble enzyme: pH and temperature dependence of the activity, self-cleavage activity, and possibility of repeated use. alpha-Chymotrypsin immobilized to magnetic bead cellulose was usedfor the proteolytic digestion of porcine pepsin A and human gastric juice and a possibility of direct use of enzyme reaction products for matrix-assisted laser desorption/ionization time of flight mass spectrometry analysis was shown.

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CE - Biochemistry

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)<br>Z - Vyzkumny zamer (s odkazem do CEZ)

Publication year

2012

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Applied biochemistry and biotechnology

ISSN

0273-2289

e-ISSN

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Volume of the periodical

168

Issue of the periodical within the volume

2

Country of publishing house

US - UNITED STATES

Number of pages

11

Pages from-to

295-305

UT code for WoS article

000309224300007

EID of the result in the Scopus database

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