Immobilized endoproteinase Glu-C to magnetic bead cellulose as a tool in proteomic analysis

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F13%3A10189373" target="_blank" >RIV/00216208:11110/13:10189373 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/13:10189373
Result on the web
<a href="http://dx.doi.org/10.1002/jssc.201300118" target="_blank" >http://dx.doi.org/10.1002/jssc.201300118</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1002/jssc.201300118" target="_blank" >10.1002/jssc.201300118</a>

Result language
angličtina
Original language name
Immobilized endoproteinase Glu-C to magnetic bead cellulose as a tool in proteomic analysis
Original language description
Magnetic bead cellulose activated with divinyl sulfone was used for the immobilization of Staphylococcus aureus endoproteinase Glu-C (EC 3.4.21.19). The immobilized proteinase was characterized by increased thermostability, by decreased self-cleavage activity, and a possibility of repeated use. The prepared immobilized enzyme was applied for the proteolytic cleavage of -casein and BSA under different conditions (different composition of buffers, different pH, and different time of digestion). The possibilities of the direct use of enzyme reaction products for MALDI TOF MS analysis were shown.
Czech name
—
Czech description
—

Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
CB - Analytical chemistry, separation
OECD FORD branch
—

Project
<a href="/en/project/GAP206%2F12%2F0381" target="_blank" >GAP206/12/0381: Development of ultrasensitive electrochemical immunosensors for early detection of clinically significant biomarkers of malignant diseases</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Similar results(10)