DNaseI immobilized on magnetic hydrogel particles in DNA analysis
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216224%3A14310%2F00%3A00003592" target="_blank" >RIV/00216224:14310/00:00003592 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
DNaseI immobilized on magnetic hydrogel particles in DNA analysis
Original language description
A simple separation of enzymes from reaction mixture can be achieved using enzymes immobilized on magnetic carriers. The aim of our work was to utilize enzymes immobilized on magnetic carriers for isolation and purification of chromosomal and plasmid DNAs. Properties of DNaseI immobilized on both magnetic bead cellulose and poly(2-hydroxyethyl methacrylate)microspheres were studied. Activities of free and immobilized DNaseI were studied in dependence on pH and the presence of activating bivalent cations. Moreover, biological activity of DNaseI immobilized on both magnetic supports was tested by cleavage of plasmid pUC19 DNA.
Czech name
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Czech description
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Classification
Type
D - Article in proceedings
CEP classification
EI - Biotechnology and bionics
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)
Others
Publication year
2000
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Article name in the collection
20th International Symposium on the Separation and Analysis of Proteins, Peptides and Polynucleotides
ISBN
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ISSN
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e-ISSN
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Number of pages
1
Pages from-to
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Publisher name
University of Ljubljana
Place of publication
Ljubljana
Event location
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Event date
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Type of event by nationality
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UT code for WoS article
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