Microfiltration method of removal of bacterial contaminants and their monitoring by nitric oxide and Limulus assays
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F13%3A10138963" target="_blank" >RIV/00216208:11110/13:10138963 - isvavai.cz</a>
Alternative codes found
RIV/68378041:_____/13:00382480 RIV/61388963:_____/13:00382480 RIV/00216208:11140/13:10138963
Result on the web
<a href="http://dx.doi.org/10.1016/j.niox.2012.08.078" target="_blank" >http://dx.doi.org/10.1016/j.niox.2012.08.078</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.niox.2012.08.078" target="_blank" >10.1016/j.niox.2012.08.078</a>
Alternative languages
Result language
angličtina
Original language name
Microfiltration method of removal of bacterial contaminants and their monitoring by nitric oxide and Limulus assays
Original language description
Similar to lipopolysaccharide (LPS), a product of Gram-negative bacteria, the signal macromolecules of Gram-positive bacteria lipoteichoic acid (LTA) and peptidoglycan (PGN) possess multiple biological activities. They may be a source of misinterpretation of experimental findings. We have found that not only LPS but also LTA and PGN can be detected by the Limulus amebocyte lysate (LAL) assay. All of them stimulate the high output in vitro nitric oxide (NO) production of in rat peritoneal cells. The onset of the NO enhancement was observed with 25-100 pg/ml of LPS and 25-100 ng/ml of PGN and LTA. Polymyxin B (PMX), if applied at concentration 10,000-fold higher than that of LPS, can completely inhibit the NO and LAL binding responses of LPS. The NO-stimulatory and LAL-binding properties of LTA and PGN are not eliminated by PMX. Handling of LPS contamination with PMX may be associated with serious problems because it possesses intrinsic biological activity and becomes cytotoxic at concen
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FR - Pharmacology and apothecary chemistry
OECD FORD branch
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Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2013
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nitric Oxide - Biology and Chemistry
ISSN
1089-8603
e-ISSN
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Volume of the periodical
28
Issue of the periodical within the volume
Jan 15
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
1-7
UT code for WoS article
000315065900001
EID of the result in the Scopus database
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