Localized movement and morphology of UBF1-positive nucleolar regions are changed by gamma-irradiation in G2 phase of the cell cycle
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F15%3A10297886" target="_blank" >RIV/00216208:11110/15:10297886 - isvavai.cz</a>
Alternative codes found
RIV/00216224:14330/15:00080738
Result on the web
<a href="http://dx.doi.org/10.1080/19491034.2015.1075111" target="_blank" >http://dx.doi.org/10.1080/19491034.2015.1075111</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1080/19491034.2015.1075111" target="_blank" >10.1080/19491034.2015.1075111</a>
Alternative languages
Result language
angličtina
Original language name
Localized movement and morphology of UBF1-positive nucleolar regions are changed by gamma-irradiation in G2 phase of the cell cycle
Original language description
The nucleolus is a well-organized site of ribosomal gene transcription. Moreover, many DNA repair pathway proteins, including ATM, ATR kinases, MRE11, PARP1 and Ku70/80, localize to the nucleolus (Moore et al., 2011). We analyzed the consequences of DNAdamage in nucleoli following ultraviolet A (UVA), C (UVC), or gamma-irradiation in order to test whether and how radiation-mediated genome injury affects local motion and morphology of nucleoli. Because exposure to radiation sources can induce changes inthe pattern of UBF1-positive nucleolar regions, we visualized nucleoli in living cells by GFP-UBF1 expression for subsequent morphological analyses and local motion studies. UVA radiation, but not 5 Gy of gamma-rays, induced apoptosis as analyzed by anadvanced computational method. In non-apoptotic cells, we observed that gamma-radiation caused nucleolar re-positioning over time and changed several morphological parameters, including the size of the nucleolus and the area of individual
Czech name
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Czech description
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Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
EB - Genetics and molecular biology
OECD FORD branch
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Result continuities
Project
<a href="/en/project/GBP302%2F12%2FG157" target="_blank" >GBP302/12/G157: Dynamics and Organization of Chromosomes in the Cell Cycle and during Differentiation under Normal and Pathological Conditions</a><br>
Continuities
O - Projekt operacniho programu
Others
Publication year
2015
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Nucleus
ISSN
1949-1034
e-ISSN
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Volume of the periodical
6
Issue of the periodical within the volume
4
Country of publishing house
US - UNITED STATES
Number of pages
13
Pages from-to
301-313
UT code for WoS article
000360666000008
EID of the result in the Scopus database
2-s2.0-84954480958