Localized movement and morphology of UBF1-positive nucleolar regions are changed by -irradiation in G2 phase of the cell cycle

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F68081707%3A_____%2F15%3A00456708" target="_blank" >RIV/68081707:_____/15:00456708 - isvavai.cz</a>

Result on the web

<a href="http://dx.doi.org/10.1080/19491034.2015.1075111" target="_blank" >http://dx.doi.org/10.1080/19491034.2015.1075111</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1080/19491034.2015.1075111" target="_blank" >10.1080/19491034.2015.1075111</a>

Result language

angličtina

Original language name

Localized movement and morphology of UBF1-positive nucleolar regions are changed by -irradiation in G2 phase of the cell cycle

Original language description

The nucleolus is a well-organized site of ribosomal gene transcription. Moreover, many DNA repair pathway proteins, including ATM, ATR kinases, MRE11, PARP1 and Ku70/80, localize to the nucleolus (Moore etal., 2011). We analyzed the consequences of DNA damage in nucleoli following ultraviolet A (UVA), C (UVC), or -irradiation in order to test whether and how radiation-mediated genome injury affects local motion and morphology of nucleoli. Because exposure to radiation sources can induce changes in the pattern of UBF1-positive nucleolar regions, we visualized nucleoli in living cells by GFP-UBF1 expression for subsequent morphological analyses and local motion studies. UVA radiation, but not 5Gy of -rays, induced apoptosis as analyzed by an advanced computational method. In non-apoptotic cells, we observed that -radiation caused nucleolar re-positioning over time and changed several morphological parameters, including the size of the nucleolus and the area of individual UBF1-positive fo

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

BO - Biophysics

OECD FORD branch

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Project

Result was created during the realization of more than one project. More information in the Projects tab.

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Nucleus

ISSN

1949-1034

e-ISSN

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Volume of the periodical

6

Issue of the periodical within the volume

4

Country of publishing house

US - UNITED STATES

Number of pages

13

Pages from-to

301-313

UT code for WoS article

000360666000008

EID of the result in the Scopus database

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