Histone deacetylase inhibitors in plasma cell leukemia treatment: effect of bone marrow microenvironment

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F17%3A10362309" target="_blank" >RIV/00216208:11110/17:10362309 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/17:10362309 RIV/00064190:_____/17:N0000083 RIV/00064165:_____/17:10362309

Result on the web

<a href="http://dx.doi.org/10.4149/neo_2017_209" target="_blank" >http://dx.doi.org/10.4149/neo_2017_209</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.4149/neo_2017_209" target="_blank" >10.4149/neo_2017_209</a>

Result language

angličtina

Original language name

Histone deacetylase inhibitors in plasma cell leukemia treatment: effect of bone marrow microenvironment

Original language description

In the presented study we analysed the effect of histone deacetylase inhibitors (HDACi) suberoylanilide hydroxamic acid (SAHA) and valproic acid (VPA) on human plasma cell leukemia (PCL) cell line UHKT-944 in the presence of bone marrow microenvironment (BMM). For the analysis, the cells were cultured alone, with bone marrow stromal cells (BMSCs), with extracellular matrix (ECM) components or with interleukin-6, and treated with varied concentrations of SAHA and VPA for 24/48 hours. To study the effect of HDACi, we investigated cell proliferation, apoptosis, cell cycle and changes in selected signalling pathways. We found that both SAHA and VPA induced apoptosis, but had no effect on the cell cycle distribution of UHKT-944 cells. Investigation of the antiproliferative effect of SAHA and VPA revealed that BMSCs and high concentration of interleukin-6 had partial protective effect against SAHA or both inhibitors, respectively. No effect of ECM components on the efficiency of HDACi was observed. We further revealed that VPA down-regulated STAT3 phosphorylation while both inhibitors decreased Akt phosphorylation. In conclusion, VPA and SAHA might represent an additional therapeutic strategy in the PCL treatment. Protective effect of BMM should be taken into account when investigating prospective therapeutic agents against plasma cell disorders.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30204 - Oncology

Project

—

Continuities

V - Vyzkumna aktivita podporovana z jinych verejnych zdroju

Publication year

2017

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Neoplasma

ISSN

0028-2685

e-ISSN

—

Volume of the periodical

64

Issue of the periodical within the volume

2

Country of publishing house

SK - SLOVAKIA

Number of pages

12

Pages from-to

228-239

UT code for WoS article

000402091900009

EID of the result in the Scopus database

2-s2.0-85020455643