Immunosuppressive protocols with tacrolimus after cryopreserved aortal allotransplantation in rats

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F18%3A10378504" target="_blank" >RIV/00216208:11110/18:10378504 - isvavai.cz</a>

Alternative codes found

RIV/00179906:_____/18:10378504 RIV/00064165:_____/18:10378504

Result on the web

<a href="https://doi.org/10.1371/journal.pone.0201984" target="_blank" >https://doi.org/10.1371/journal.pone.0201984</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1371/journal.pone.0201984" target="_blank" >10.1371/journal.pone.0201984</a>

Result language

angličtina

Original language name

Immunosuppressive protocols with tacrolimus after cryopreserved aortal allotransplantation in rats

Original language description

Objectives and design: The aim of our study was to simulate in rats all aspects and techniques used in our new clinical program of cryopreserved alloarterial transplantation and investigate the influence of two immunosuppressive protocols with tacrolimus on acute rejection of these allografts. Materials and methods: Cryopreserved abdominal aortic grafts were transplanted between Brown-Norway and Lewis rats. Tacrolimus (0.2 mg/kg daily) was administered from day 1 to day 30 (TAC1) or from day 7 to day 30 (TAC7), respectively. No immunosuppressed isogeneic (ISO) and allogeneic (ALO) rats combination served as control. Aortal wall infiltration by immunocompetent cells (MHC II+ cells of recipient origin) was studied on day 30 after transplantation. Flow cytometry was used for the analysis of day 30 sera for the presence of donor specific antiMHC class I and II antibodies. Results: The aortal allografts in both immunosuppressed groups showed regular morphology of aortal wall with no depositions of immunoglobulin G on day 30. The adventitial infiltration of non-immunosuppressed aortal allografts by MHC class II positive cells of recipient origin was significantly higher (ALO 20.7 +/- 6.7 cells, P&lt;0.001) compared to both immunosuppressed groups (TAC1 5.9 +/- 5.5 cells, TAC7 6.1 +/- 5.1 cells). Day 30 sera from the allogeneic non-immunosuppressed animals decreased significantly the binding of fluorescence labelled MHC class I (46.9 +/- 19.4%) and class II (65.8 +/- 11.9%) antibody to donors spleen cells compared with day 30 sera from both immunosuppressed groups (TAC1, anti-MHC class 1102.4 +/- 4.2%, p &lt; 0.001, anti-MHC class II 102.6 +/- 6.0%), (TAC7, anti-MHC class I 79.9 +/- 3.3%, p &lt; 0.001, anti-MHC class II 80.9 +/- 2.7%). Conclusion: Both immunosuppressed protocols with tacrolimus (administration from day 1 or from day 7 following transplantation) were able to suppress acute cell- and antibody-mediated rejection of cryopreserved abdominal aortic allografts processed in accordance with our new standardized clinical protocol.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

30201 - Cardiac and Cardiovascular systems

Project

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Continuities

S - Specificky vyzkum na vysokych skolach<br>I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

PLoS One

ISSN

1932-6203

e-ISSN

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Volume of the periodical

13

Issue of the periodical within the volume

8

Country of publishing house

US - UNITED STATES

Number of pages

17

Pages from-to

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UT code for WoS article

000441232600075

EID of the result in the Scopus database

2-s2.0-85052315026