The response of C19-and some C21-steroids during Synacthen and insulin tolerance test

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F18%3A10382018" target="_blank" >RIV/00216208:11110/18:10382018 - isvavai.cz</a>

Alternative codes found

RIV/00023761:_____/18:N0000025 RIV/00064165:_____/18:10382018

Result on the web

<a href="https://doi.org/10.1016/j.steroids.2018.01.009" target="_blank" >https://doi.org/10.1016/j.steroids.2018.01.009</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1016/j.steroids.2018.01.009" target="_blank" >10.1016/j.steroids.2018.01.009</a>

Result language

angličtina

Original language name

The response of C19-and some C21-steroids during Synacthen and insulin tolerance test

Original language description

Testing of the adrenal function with ACTH 1-24 (Synacthen test) or insulin (insulin tolerance test-ITT) is commonly used. The question of ongoing debate is the dose of Synacthen. Moreover, it may be important from the physiological point of view besides measurement of cortisol levels and 17 alpha-hydroxy-progesterone to know also the response of other steroids to these test. The plasma levels of 24 free steroids and their polar conjugates were followed after stimulation of 1 mu g, 10 mu g and 250 mu g of ACTH 1-24 and after insulin administration in thirteen healthy subjects. The study aimed to describe a response of steroid metabolome to various doses of ACTH 1-24 and to find the equivalency of these tests. The additional ambition was to contribute to understanding of physiology of these stimulation tests and suggest an additional marker for HPA axis evaluation. No increase of most conjugated steroids and even decrease of some of them during all of the Synacthen tests and ITT at 60th mM were observed. The levels of steroid conjugates decreased in ITT but did not during all of the Synacthen tests by 20 mM of each test. Testosterone and estradiol did not increase during the Synacthen tests or ITT as expected. The results suggest that the conjugated steroids in the circulation can serve as reserve stock for rapid conversion into free steroids in the first minutes of the stress situation. Various doses of ACTH 1-24 used in the Synacthen tests implicate earlier or later occurrence of maximal response of stimulated steroids. The equivalent dose to ITT and standard 250 mu g of ACTH 1-24 seemed to be dose of 10 mu g ACTH 1-24 producing the similar response in all of the steroids in the 60th min of the test.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

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OECD FORD branch

10608 - Biochemistry and molecular biology

Project

—

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Steroids

ISSN

0039-128X

e-ISSN

—

Volume of the periodical

139

Issue of the periodical within the volume

November

Country of publishing house

US - UNITED STATES

Number of pages

7

Pages from-to

53-59

UT code for WoS article

000448101100007

EID of the result in the Scopus database

2-s2.0-85045438164