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Biophysical Methods to Analyze Direct G-Protein Regulation of Neuronal Voltage-Gated Calcium Channels

The result's identifiers

  • Result code in IS VaVaI

    <a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11110%2F21%3A10441267" target="_blank" >RIV/00216208:11110/21:10441267 - isvavai.cz</a>

  • Result on the web

    <a href="https://doi.org/10.1007/978-1-0716-1522-5_26" target="_blank" >https://doi.org/10.1007/978-1-0716-1522-5_26</a>

  • DOI - Digital Object Identifier

    <a href="http://dx.doi.org/10.1007/978-1-0716-1522-5_26" target="_blank" >10.1007/978-1-0716-1522-5_26</a>

Alternative languages

  • Result language

    angličtina

  • Original language name

    Biophysical Methods to Analyze Direct G-Protein Regulation of Neuronal Voltage-Gated Calcium Channels

  • Original language description

    Neuronal voltage-gated calcium channels play an essential role for calcium entry into presynaptic endings responsible for the release of neurotransmitters. In turn, and in order to fine-tune synaptic activity, numerous neurotransmitters exert a potent negative feedback over the calcium signal provided by G-protein-coupled receptors that can be recognized by characteristic biophysical modifications of the calcium current. There are two main biophysical approaches to analyze direct G-protein regulation of voltage-gated calcium channels: the so-called &quot;double pulse&quot; method, which is indirectly assessed by the gain of current produced by a depolarizing prepulse potential, and the &quot;subtraction&quot; method that allows the analysis of G-protein regulation from the ionic currents induced by regular depolarizing pulses. The later method separates the ionic currents due to nonregulated channels from the ion currents that result from a progressive departure of G-proteins from regulated channels, thereby providing valuable information on the OFF kinetics of G-protein regulation. In this chapter, we introduce these &quot;double pulses&quot; and &quot;subtraction&quot; procedures for use primarily with single cells, and also discuss the limitations inherent to these two approaches.

  • Czech name

  • Czech description

Classification

  • Type

    C - Chapter in a specialist book

  • CEP classification

  • OECD FORD branch

    30105 - Physiology (including cytology)

Result continuities

  • Project

  • Continuities

    I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Others

  • Publication year

    2021

  • Confidentiality

    S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Data specific for result type

  • Book/collection name

    Receptor and Ion Channel Detection in the Brain

  • ISBN

    978-1-07-161521-8

  • Number of pages of the result

    11

  • Pages from-to

    429-439

  • Number of pages of the book

    554

  • Publisher name

    Humana

  • Place of publication

    New York

  • UT code for WoS chapter