Biophysical Methods to Analyze Direct G-Protein Regulation of Neuronal Voltage-Gated Calcium Channels
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F16%3A00459795" target="_blank" >RIV/61388963:_____/16:00459795 - isvavai.cz</a>
Result on the web
<a href="http://dx.doi.org/10.1007/978-1-4939-3064-7_22" target="_blank" >http://dx.doi.org/10.1007/978-1-4939-3064-7_22</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1007/978-1-4939-3064-7_22" target="_blank" >10.1007/978-1-4939-3064-7_22</a>
Alternative languages
Result language
angličtina
Original language name
Biophysical Methods to Analyze Direct G-Protein Regulation of Neuronal Voltage-Gated Calcium Channels
Original language description
Neuronal voltage-gated calcium channels play an essential role for calcium entry into presynaptic endings responsible for the release of neurotransmitters. In turn, and in order to fine tune synaptic activity, numerous neurotransmitters exert a potent negative feedback over the calcium signal provided by G-protein-coupled receptors that can be recognized by characteristic biophysical modifications of the calcium current. There are two main biophysical approaches to analyze direct G-protein regulation of voltage-gated calcium channels: the so-called double-pulse method, which is indirectly assessed by the gain of current produced by a depolarizing prepulse potential, and the "subtraction" method that allows the analysis of G-protein regulation from the ionic currents induced by regular depolarizing pulses. The later method separates the ionic currents due to nonregulated channels from the ion currents that result from a progressive departure of G-proteins from regulated channels, thereby providing valuable information on the OFF kinetics of G-protein regulation. In this chapter, we introduce these "double pulses" and "subtraction" procedures for use primarily with single cells and also discuss the limitations inherent to these two approaches.
Czech name
—
Czech description
—
Classification
Type
C - Chapter in a specialist book
CEP classification
CE - Biochemistry
OECD FORD branch
—
Result continuities
Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace
Others
Publication year
2016
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Book/collection name
Receptor and Ion Channel Detection in the Brain: Methods and Protocols
ISBN
978-1-4939-3063-0
Number of pages of the result
12
Pages from-to
357-368
Number of pages of the book
475
Publisher name
Humana Press
Place of publication
New York
UT code for WoS chapter
—