Inhibition of cell growth, induction of apoptosis and mechanism of action of the novel platinum compound cis-diaminechloro-[2-(diethylamino) ethyl 4-amino-benzoate, N-4]-chloride platinum (II) monohydrochloride monohydrate
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F04%3A00000966" target="_blank" >RIV/00216208:11120/04:00000966 - isvavai.cz</a>
Result on the web
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DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
Inhibition of cell growth, induction of apoptosis and mechanism of action of the novel platinum compound cis-diaminechloro-[2-(diethylamino) ethyl 4-amino-benzoate, N-4]-chloride platinum (II) monohydrochloride monohydrate
Original language description
Cis-diaminechloro-[2-(diethylamino) ethyl 4-amino-benzoate, N-4]-chloride platinum (II) monohydrochloride monohydrate (DPR) is a new platinum triamine complex obtained from the synthesis of cisplatin and procaine. In this paper we analyzed, adopting a disease-oriented strategy, the tumour selectivity of this compound, its ability to induce apoptosis and its mechanism of interaction with DNA. The inhibition of cell proliferation was evaluated by the MTT assay using a panel of 51 tumour cell lines. Some of them were also evaluated for the induction of apoptosis by 4'-6-diamidine-2'-phenylindole (DAPI) staining, Western blot of p53 protein and agarose gel electrophoresis of ladder DNA. Finally, interstand cross-links (ISCL) were evaluated by ethidium bromide fluorescence technique. When evaluated by the MTT assay, DPR showed a high selective activity for neuroblastoma, small cell lung cancer (SCLC), ovarian cancer and leukemia cell lines. The comparison of mean graphs of DPR and cisplatin
Czech name
Inhibice růstu buněk, indukce apoptózy a mechanizmus účinku nové platinové sloučeniny cis-diaminechloro-2-diethylamino ethyl 4-amino-benzoát, N 4- chloridu platiny (II) monohydrochlorid monohydrátu
Czech description
Protinádorový účinek nové platinové sloučeniny získané syntézou cisplatiny a prokainu, komplexu cis-diaminechloro-2-diethylamino ethyl 4-amino-benzoát, N 4- chloridu platiny (II) monohydrochlorid monohydrátu, byl sledován v nádorových buňkách kultivovaných in vitro. Získané výsledky prokázaly protinádorové působení nové sloučeniny, která indukovala apoptózu v nádorových buňkách, zejména neuroblastomu a malobuněčného karcinomu.
Classification
Type
J<sub>x</sub> - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)
CEP classification
FD - Oncology and haematology
OECD FORD branch
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Result continuities
Project
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Continuities
S - Specificky vyzkum na vysokych skolach
Others
Publication year
2004
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Investigational New Drugs
ISSN
0167-6997
e-ISSN
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Volume of the periodical
22
Issue of the periodical within the volume
1
Country of publishing house
NL - THE KINGDOM OF THE NETHERLANDS
Number of pages
14
Pages from-to
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UT code for WoS article
000186895600001
EID of the result in the Scopus database
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