Parallel optimization and genotyping of multiple single-nucleotide polymorphism markers by sample pooling approach using cycling-gradient CE with multiple injections

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F06%3A00000105" target="_blank" >RIV/00216208:11120/06:00000105 - isvavai.cz</a>

Alternative codes found

RIV/00023752:_____/06:00000522 RIV/26475821:_____/06:#0000007

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

Parallel optimization and genotyping of multiple single-nucleotide polymorphism markers by sample pooling approach using cycling-gradient CE with multiple injections

Original language description

Increasing importance of single-nucleotide polymorphisms (SNPs) in determination of disease susceptibility or in prediction of therapy response brings attention of many molecular diagnostic laboratories to simple and low-cost SNP genotyping methodologies. We have recently introduced a mutation detection technique based on analysis of homo- and heteroduplex PCR fragments resolved in cycling temperature gradient conditions on a conventional multicapillary-array DNA sequencer. The main advantage of this technique is in its simplicity with no requirement for sample cleanup prior to the analysis. In this report we present a practical application of the technology for genotyping of SNP markers in two separate clinical projects resulting in a combined set of44 markers screened in over 500 patients. Initially, a design of PCR primers and conditions was performed for each SNP marker. Then, optimization of CE running conditions (limited just to the proper selection of temperature cycling) was p

Czech name

Parallel optimization and genotyping of multiple single-nucleotide polymorphism markers by sample pooling approach using cycling-gradient CE with multiple injections

Czech description

Paralelní optimalizace a genotypizace mnohočetných markerů u jednoduchých nukleotidových polymorfismů za použití "cycling-gradient CE" s mnohočetnou injektáží.

Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

EB - Genetics and molecular biology

OECD FORD branch

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Project

<a href="/en/project/NR8039" target="_blank" >NR8039: Identification of inherited DNA polymorphisms in genes of testosterone synthetic pathway as factors of genetic predisposition to development of prostate cancer and their use in prevention and prognosis in Czech population</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2006

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

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Volume of the periodical

27

Issue of the periodical within the volume

19

Country of publishing house

DE - GERMANY

Number of pages

8

Pages from-to

3856-3863

UT code for WoS article

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EID of the result in the Scopus database

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