Rapid determination of branched chain amino acids in human blood plasma by pressure assisted capillary electrophoresis with contactless conductivity detection

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F15%3A43909342" target="_blank" >RIV/00216208:11120/15:43909342 - isvavai.cz</a>

Alternative codes found

RIV/00064173:_____/15:#0000489

Result on the web

<a href="http://dx.doi.org/10.1002/elps.201400585" target="_blank" >http://dx.doi.org/10.1002/elps.201400585</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/elps.201400585" target="_blank" >10.1002/elps.201400585</a>

Result language

angličtina

Original language name

Rapid determination of branched chain amino acids in human blood plasma by pressure assisted capillary electrophoresis with contactless conductivity detection

Original language description

A capillary electrophoresis (CE) method with contactless conductivity detection has been developed for the clinical determination of the branched chain amino acids (BCAAs) valine, isoleucine, and leucine in human blood plasma. The CE separation was performed in an optimized background electrolyte with composition 3.2 M acetic acid in 20% v/v methanol, pH 2.0. The achieved separation time was 125 s when using a capillary with an effective length of 14.7 cm, electric field intensity of 0.96 kV/cm and simultaneous application of a hydrodynamic pressure of 50 mbar. The separation efficiency in blood plasma equalled 461 000 theoretical plates/m for valine and isoleucine, and 455 000 theoretical plates/m for leucine; the detection limits are equal to 0.4 ?Mfor all three amino acids. The relative standard deviation values for repeatability of the migration time equalled 0.1% for measurements during a single day and 0.3% for measurements on different days; the relative standard deviation valu

Czech name

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Czech description

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Type

J_x - Unclassified - Peer-reviewed scientific article (Jimp, Jsc and Jost)

CEP classification

CB - Analytical chemistry, separation

OECD FORD branch

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Project

<a href="/en/project/NT14416" target="_blank" >NT14416: Effect of branched-chain amino acids and saturated fatty acids on development of insulin resistance</a><br>

Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2015

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Electrophoresis

ISSN

0173-0835

e-ISSN

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Volume of the periodical

36

Issue of the periodical within the volume

16

Country of publishing house

DE - GERMANY

Number of pages

7

Pages from-to

1969-1975

UT code for WoS article

000360055300024

EID of the result in the Scopus database

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