Measuring venous-arterial differences of valine, isoleucine, leucine, alanine and glutamine in skeletal muscles using counter-current electrophoresis with contactless conductivity detection

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388963%3A_____%2F20%3A00519392" target="_blank" >RIV/61388963:_____/20:00519392 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11120/20:43919519 RIV/00064173:_____/20:N0000045
Result on the web
<a href="https://www.sciencedirect.com/science/article/pii/S1572665719310409?via%3Dihub" target="_blank" >https://www.sciencedirect.com/science/article/pii/S1572665719310409?via%3Dihub</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jelechem.2019.113772" target="_blank" >10.1016/j.jelechem.2019.113772</a>

Result language
angličtina
Original language name
Measuring venous-arterial differences of valine, isoleucine, leucine, alanine and glutamine in skeletal muscles using counter-current electrophoresis with contactless conductivity detection
Original language description
Plasmatic levels of valine, isoleucine, leucine, alanine and glutamine were determined by capillary electrophoresis with contactless conductivity detection. A new kind of covalently coated capillary based on polyacrylamide and (3-acrylamidopropyl) trimethylammonium chloride was employed. The amino acids were separated in the counter-current regime, in which the analytes migrate under counter-current electro-osmotic flow with a value of 5.53 · 10−9 m2 V−1 s−1 in the background electrolyte, 3.2 mol/L AcOH +10% v/v MeOH. The baseline separation of all five amino acids from other plasmatic compounds was achieved in <5 min, the limits of detections are 0.13–0.14 μmol/L for the treated sample and 0.50–0.58 μmol/L for the untreated plasma. The method was used to measure venous-arterial differences of amino acids in hyperinsulinemic patients with cachexia.
Czech name
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Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10406 - Analytical chemistry

Project
Result was created during the realization of more than one project. More information in the Projects tab.
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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