HPLC-ED/UV for determination of vanillylmandelic acid in human urine after solid phase extraction

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F18%3A00498465" target="_blank" >RIV/61388955:_____/18:00498465 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/18:10409569

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

HPLC-ED/UV for determination of vanillylmandelic acid in human urine after solid phase extraction

Original language description

HPLC with electrochemical and spectrophotometric detection (ED/UV) after solid phase extraction (SPE) was used for determination of vanillylmandelic acid in human urine. HPLC-ED was performed at a glassy carbon electrode in a “wall-jet” arrangement in acetate-phosphate buffer at pH = 2.5 and gradient elution (increasing content ofnacetonitrile from 5 to 25% in 10 minutes) was used. Optimized parameters were following: flow rate of mobile phase 1 mL min−1, detection potential +1.1 V, detection wavelength 279 nm, injected volume 20 μL. Dependence of the peak current on the analyte concentration was linear in the concentration range from 10 to 150 μmol L−1, with obtained limits of detection 2.6 μmol L−1 (calculated from peak height) and 1.9 μmol L−1 (calculated from peak area) for HPLC-ED, and 11.0 μmol L−1 (calculated from peak height) and 9.8 μmol L−1 (calculated from peak area) for HPLC-UV.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project

<a href="/en/project/GA17-03868S" target="_blank" >GA17-03868S: New Methods of Electrochemical Monitoring of Biologically Active Organic Compounds in Environmental, Biological and Food Matrices</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

Proceedings of the 14th International Students Conference “Modern Analytical Chemistry”

ISBN

978-80-7444-059-5

ISSN

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e-ISSN

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Number of pages

5

Pages from-to

82-86

Publisher name

Univerzita Karlova v Praze, Přírodovědecká fakulta

Place of publication

Praha

Event location

Praha

Event date

Sep 20, 2018

Type of event by nationality

EUR - Evropská akce

UT code for WoS article

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