HPLC-ED/UV with Solid Phase Extraction for the Determination of 5-Hydroxyindole-3-acetic Acid

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10375980" target="_blank" >RIV/00216208:11310/18:10375980 - isvavai.cz</a>
Alternative codes found
RIV/61388955:_____/18:00490024
Result on the web
<a href="https://www.bestservis.eu/images/file/Sbornik_metody18_pdf/Sbornik_metody18.pdf" target="_blank" >https://www.bestservis.eu/images/file/Sbornik_metody18_pdf/Sbornik_metody18.pdf</a>
DOI - Digital Object Identifier
—

Result language
angličtina
Original language name
HPLC-ED/UV with Solid Phase Extraction for the Determination of 5-Hydroxyindole-3-acetic Acid
Original language description
Tumour biomarker, 5-hydroxyindole-3-acetic acid, has been determined in human urine using HPLC with electrochemical (at a glassy carbon electrode in a "wall-jet" arrangement) and spectrophotometric detection (ED/UV) with solid phase extraction (SPE). Mobile phase was acetate-phosphate buffer at pH 2.5, gradient elution (increasing content of acetonitrile from 5 to 25% in 10 minutes) was used, flow rate of mobile phase was set up to 1 ml.minMINUS SIGN 1, detection potential to +1.1 V and detection wavelength of 279 nm. Injected volume was 20 μl. Dependence of the peak current on the analyte concentration was linear in the whole tested concentration range (from 10 to 150 μmol.l-1) and obtained limits of detection (LODs) for HPLC-ED were 0.85 μmol.l-1 (calculated from peak height) and 0.72 μmol.l-1 (calculated from peak area). For HPLC-UV, LODs were 3.30 μmol.l-1 (calculated from peak height) and 2.25 μmol.l-1 (calculated from peak area).
Czech name
—
Czech description
—

Project
<a href="/en/project/GA17-03868S" target="_blank" >GA17-03868S: New Methods of Electrochemical Monitoring of Biologically Active Organic Compounds in Environmental, Biological and Food Matrices</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
XXXVIII. Moderní Elektrochemické Metody: Sborník přednášek
ISBN
978-80-905221-6-9
ISSN
—
e-ISSN
—
Number of pages
4
Pages from-to
147-150
Publisher name
Srsenová Lenka - Best servis Ústí nad Labem
Place of publication
Ústí nad Labem
Event location
Jetrichovice, Česká republika
Event date
May 21, 2018
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000464596500033

Similar results(10)