HPLC-ED/UV with Solid Phase Extraction for the Determination of 5-Hydroxyindole-3-acetic Acid

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F18%3A00490024" target="_blank" >RIV/61388955:_____/18:00490024 - isvavai.cz</a>

Alternative codes found

RIV/00216208:11310/18:10375980

Result on the web

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DOI - Digital Object Identifier

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Result language

angličtina

Original language name

HPLC-ED/UV with Solid Phase Extraction for the Determination of 5-Hydroxyindole-3-acetic Acid

Original language description

Determination of 5-HIAA in human urine was successfully performed using HPLC-ED/UV with SPE. HPLC-ED is a very sensitive technique, presented method does not need any sample pre-treatment apart from SPE procedure, which replaced filtration of samples of human urine to avoid problems with clogging a HPLC column. The found nativenconcentrations of 5-HIAA in human urine using HPLC-ED with SPE fully corresponds with published normal concentrations in urine (17.8 - 58.3 micromol l-1). The found native concentrations of 5-HIAA were 18.4 micromol L-1 (calculated from peak height). The presented method is fast and could be used for simplenscreening of human urine, because HPLC-ED/UV allows simultaneous determination of 5-HIAA, HVA, and VMA as important tumour biomarkers.

Czech name

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Czech description

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Type

D - Article in proceedings

CEP classification

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OECD FORD branch

10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project

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Continuities

I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year

2018

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection

XXXVIII. Moderní elektrochemické metody. Sborník přednášek

ISBN

978-80-905221-6-9

ISSN

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e-ISSN

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Number of pages

4

Pages from-to

147-150

Publisher name

Best Servis

Place of publication

Ústí nad Labem

Event location

Jetřichovice

Event date

May 21, 2018

Type of event by nationality

WRD - Celosvětová akce

UT code for WoS article

000464596500033