Simultaneous determination of tumour biomarkers homovanillic acid, vanillylmandelic acid, and 5-hydroxyindole-3-acetic acid in human urine using single run HPLC with a simple wall-jet glassy carbon electrochemical detector

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F61388955%3A_____%2F20%3A00532429" target="_blank" >RIV/61388955:_____/20:00532429 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11310/20:10419745
Result on the web
<a href="http://hdl.handle.net/11104/0310923" target="_blank" >http://hdl.handle.net/11104/0310923</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1016/j.jelechem.2020.114629" target="_blank" >10.1016/j.jelechem.2020.114629</a>

Result language
angličtina
Original language name
Simultaneous determination of tumour biomarkers homovanillic acid, vanillylmandelic acid, and 5-hydroxyindole-3-acetic acid in human urine using single run HPLC with a simple wall-jet glassy carbon electrochemical detector
Original language description
HPLC with amperometric detection (HPLC-ED) was used for the simultaneous determination of tumour biomarkers homovanillic acid (HVA), vanillylmandelic acid (VMA), and 5-hydroxyindole-3-acetic acid (5-HIAA) in one run in human urine using inexpensive lab-made glassy carbon wall-jet detector. Simple and fast solid phase extraction was used instead of earlier used more time consuming liquid-liquid extraction. Simultaneous determination of the three analytes in one run is fast with limits of detection at micromolar concentrations. Amperometric detection is more sensitive and selective than the spectrophotometric one. Calibration dependences are linear in the concentration range from 20 to 150 μmol·L−1. Interferences of other compounds present in urine were not observed for HPLC-ED. Concentrations of tumour biomarkers in urine of healthy volunteer found by HPLC-ED correspond with previously published normal physiological concentrations.
Czech name
—
Czech description
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Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
—
OECD FORD branch
10405 - Electrochemistry (dry cells, batteries, fuel cells, corrosion metals, electrolysis)

Project
<a href="/en/project/GA20-01589S" target="_blank" >GA20-01589S: New strategies for improving sensing properties of novel electrode materials via their surface pretreatment or modification</a><br>
Continuities
I - Institucionalni podpora na dlouhodoby koncepcni rozvoj vyzkumne organizace

Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

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