HPLC-ED/UV for determination of vanillylmandelic acid in human urine after solid phase extraction

Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11310%2F18%3A10409569" target="_blank" >RIV/00216208:11310/18:10409569 - isvavai.cz</a>
Alternative codes found
RIV/61388955:_____/18:00498465
Result on the web
<a href="https://web.natur.cuni.cz/analchem/isc-mac/" target="_blank" >https://web.natur.cuni.cz/analchem/isc-mac/</a>
DOI - Digital Object Identifier
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Result language
angličtina
Original language name
HPLC-ED/UV for determination of vanillylmandelic acid in human urine after solid phase extraction
Original language description
HPLC with electrochemical and spectrophotometric detection (ED/UV) after solid phase extraction (SPE) was used for determination of vanillylmandelic acid in human urine. HPLC-ED was performed at a glassy carbon electrode in a "wall-jet" arrangement in acetate-phosphate buffer at pH = 2.5 and gradient elution (increasing content of acetonitrile from 5 to 25% in 10 minutes) was used. Optimized parameters were following: flow rate of mobile phase 1 mL min(-1), detection potential +1.1 V, detection wavelength 279 nm, injected volume 20 mu L. Dependence of the peak current on the analyte concentration was linear in the concentration range from 10 to 150 mu mol L-1, with obtained limits of detection 2.6 mu mol L-1 (calculated from peak height) and 1.9 mu mol L-1 (calculated from peak area) for HPLC-ED, and 11.0 mu mol L-1 (calculated from peak height) and 9.8 mu mol L-1 (calculated from peak area) for HPLC-UV.
Czech name
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Czech description
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Project
<a href="/en/project/GA17-03868S" target="_blank" >GA17-03868S: New Methods of Electrochemical Monitoring of Biologically Active Organic Compounds in Environmental, Biological and Food Matrices</a><br>
Continuities
P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year
2018
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Article name in the collection
Proceedings of the 14th International Students Conference “Modern Analytical Chemistry“
ISBN
978-80-7444-059-5
ISSN
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e-ISSN
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Number of pages
5
Pages from-to
82-86
Publisher name
Univerzita Karlova, Přírodovědecká fakulta
Place of publication
Praha 2
Event location
Charles University, Praha
Event date
Sep 20, 2018
Type of event by nationality
EUR - Evropská akce
UT code for WoS article
000495572500014

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