Stearate-induced apoptosis in human pancreatic β-cells is associated with changes in membrane protein expression and these changes are inhibited by oleate

Result code in IS VaVaI

<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F19%3A43917589" target="_blank" >RIV/00216208:11120/19:43917589 - isvavai.cz</a>

Result on the web

<a href="https://doi.org/10.1002/prca.201800104" target="_blank" >https://doi.org/10.1002/prca.201800104</a>

DOI - Digital Object Identifier

<a href="http://dx.doi.org/10.1002/prca.201800104" target="_blank" >10.1002/prca.201800104</a>

Result language

angličtina

Original language name

Stearate-induced apoptosis in human pancreatic β-cells is associated with changes in membrane protein expression and these changes are inhibited by oleate

Original language description

PURPOSE: Lipotoxicity is implicated in type 2 diabetes pathogenesis. Its molecular mechanisms are not completely understood. The aim of this study is to identify new suspect proteins involved in pancreatic β-cell death induction by saturated fatty acids and its inhibition by unsaturated fatty acids. EXPERIMENTAL DESIGN: Employing 2DE analysis and subsequent western blot confirmation, we assessed the differences in membrane/membrane-associated protein expression in human β-cell line NES2Y during cell death induction by stearate and its inhibition by oleate. RESULTS: Induction of apoptosis by stearate was associated with significantly increased levels of Hsp90β, peroxiredoxin-1, and 14-3-3γ in the membrane fraction of NES2Y cells and significantly decreased levels of annexin A2, annexin A4, and reticulocalbin-2. All these changes were significantly inhibited by oleate co-application. No expression changes were detected after application of stearate together with oleate. Furthermore, the expression of reticulocalbin-2 was significantly decreased after stearate application also in the whole cell lysate. CONCLUSIONS AND CLINICAL RELEVANCE: We identified several membrane-associated proteins that could be related to pro- and anti-apoptotic signaling initiated by fatty acids in human pancreatic β-cells. As far as we know, annexin A4, reticulocalbin-2, and 14-3-3γ represent novel molecules related to the effect of fatty acids on β-cell viability.

Czech name

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Czech description

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Type

J_imp - Article in a specialist periodical, which is included in the Web of Science database

CEP classification

—

OECD FORD branch

30202 - Endocrinology and metabolism (including diabetes, hormones)

Project

<a href="/en/project/GP14-00630P" target="_blank" >GP14-00630P: Regulation of apoptosis induction by saturated and unsaturated fatty acids in pancreatic beta-cells: a proteomic approach</a><br>

Continuities

P - Projekt vyzkumu a vyvoje financovany z verejnych zdroju (s odkazem do CEP)

Publication year

2019

Confidentiality

S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů

Name of the periodical

Proteomics: Clinical Applications

ISSN

1862-8346

e-ISSN

—

Volume of the periodical

13

Issue of the periodical within the volume

4

Country of publishing house

DE - GERMANY

Number of pages

10

Pages from-to

"Article 1800104"

UT code for WoS article

000475990600020

EID of the result in the Scopus database

2-s2.0-85060917976