The effect of hyperbaric oxygen therapy on gene expression: microarray analysis on wound healing
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F20%3A43920008" target="_blank" >RIV/00216208:11120/20:43920008 - isvavai.cz</a>
Alternative codes found
RIV/60162694:G44__/20:00555834
Result on the web
<a href="https://www.uhms.org/uhm-search/uhm-journal-volume-47-2020/number-1/the-effect-of-hyperbaric-oxygen-therapy-on-gene-expression-microarray-analysis-on-wound-healing.html" target="_blank" >https://www.uhms.org/uhm-search/uhm-journal-volume-47-2020/number-1/the-effect-of-hyperbaric-oxygen-therapy-on-gene-expression-microarray-analysis-on-wound-healing.html</a>
DOI - Digital Object Identifier
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Alternative languages
Result language
angličtina
Original language name
The effect of hyperbaric oxygen therapy on gene expression: microarray analysis on wound healing
Original language description
Background: Hyperbaric oxygen (HBO2) therapy can have a positive effect on wound healing, angiogenesis and blood flow. No prior study has described the effects of HBO2 therapy and gene expression of this process. The goal of our research was to show the effects of HBO2 and its impact at the molecular level on angiogenesis, proliferation, differentiation, oxidative stress, inflammation, and extracellular matrix formation. Live animal subjects were used for simulating the process of wound healing under standard conditions and under the influence of HBO2. Methods: Two experimental groups were created using injured rabbits (N=24), one group (N=12) treated with hyperbaric therapy twice a day and one (N=12) with standard wound care management. Wounds were surgical, uninfected, and in healthy animal test subjects. We compared the whole genomic analysis of the transcriptome with the use of microarray technology at three intervals during treatment. Results: The induction of the wounds in rabbit skin increased expression of hundreds of genes in both treatment groups. The numbers of elevated and decreased genes gradually reduced as the wound healed. Gene expression analysis showed elevated expression of several genes associated with inflammation in both groups of injured animals. Genes connected to the process of angiogenesis, proliferation, differentiation, oxidative stress and extracellular matrix formation were without statistically significant changes. Conclusion: The evidence did not support that HBO2 had any significant effect on gene expression during wound healing. Additionally, there was no evidence to support that there were changes in gene expression in either treatment group.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
30216 - Dermatology and venereal diseases
Result continuities
Project
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Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
Undersea & Hyperbaric Medicine
ISSN
1066-2936
e-ISSN
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Volume of the periodical
47
Issue of the periodical within the volume
1
Country of publishing house
US - UNITED STATES
Number of pages
7
Pages from-to
31-37
UT code for WoS article
000535945500004
EID of the result in the Scopus database
2-s2.0-85082020209