Whole genome sequencing of macrolide resistant Streptococcus pneumoniae serotype 19A sequence type 416
The result's identifiers
Result code in IS VaVaI
<a href="https://www.isvavai.cz/riv?ss=detail&h=RIV%2F00216208%3A11120%2F20%3A43920315" target="_blank" >RIV/00216208:11120/20:43920315 - isvavai.cz</a>
Alternative codes found
RIV/00216208:11150/20:10412967 RIV/00179906:_____/20:10412967 RIV/75010330:_____/20:00013024 RIV/00216208:11140/20:10412967 RIV/00064173:_____/20:N0000200
Result on the web
<a href="https://doi.org/10.1186/s12866-020-01909-1" target="_blank" >https://doi.org/10.1186/s12866-020-01909-1</a>
DOI - Digital Object Identifier
<a href="http://dx.doi.org/10.1186/s12866-020-01909-1" target="_blank" >10.1186/s12866-020-01909-1</a>
Alternative languages
Result language
angličtina
Original language name
Whole genome sequencing of macrolide resistant Streptococcus pneumoniae serotype 19A sequence type 416
Original language description
BACKGROUND: The resistance of Streptococcus pneumoniae to macrolides is becoming an increasingly important issue and thus it is important to understand the genetics related to adaptation of this species to the widespread use of antibiotics in Europe. The 58 isolates of S. pneumoniae belonging to sequence type (ST) 416 and serotype 19A and to several different phenotypes originated from Italy, Portugal and Czech Republic were thus sequenced on Illumina MiSeq. The aim of the study was to describe genetical origine of isolates, investigate their macrolide resistance and suggest reasons for spread of ST416 in the Czech Republic. RESULTS: Investigation of genes associated with serotype determined serotype switch between 15B and 19A serotypes and core genome multilocus sequence typing (cgMLST) confirmed the origine of concerned isolates in Netherlands-37 clone. Inspected genomes proved variability of genes associated with the macrolide resistance even within closely genetically relative isolates. CONCLUSIONS: Participation of 19A/ST416 on the spread of Netherlands-37 is accompanied by serotype switch between 19A and 15B serotypes and with acquisition of genes involved in macrolide resistance to the clone that was originally macrolide susceptible. There is evident tendency to interchanging and modifications of these and surrounding genes, that could lead to accelerate spreading of this sequence type in regions with high macrolide consumption.
Czech name
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Czech description
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Classification
Type
J<sub>imp</sub> - Article in a specialist periodical, which is included in the Web of Science database
CEP classification
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OECD FORD branch
10606 - Microbiology
Result continuities
Project
<a href="/en/project/NV16-27109A" target="_blank" >NV16-27109A: Evolution of emergent multidrug resistant Streptococcus pneumoniae 19A serotype in era of pneumococcal vaccination.</a><br>
Continuities
V - Vyzkumna aktivita podporovana z jinych verejnych zdroju
Others
Publication year
2020
Confidentiality
S - Úplné a pravdivé údaje o projektu nepodléhají ochraně podle zvláštních právních předpisů
Data specific for result type
Name of the periodical
BMC Microbiology
ISSN
1471-2180
e-ISSN
—
Volume of the periodical
20
Issue of the periodical within the volume
July
Country of publishing house
GB - UNITED KINGDOM
Number of pages
10
Pages from-to
"Article 224"
UT code for WoS article
000555944000002
EID of the result in the Scopus database
2-s2.0-85088622988